Solar ultraviolet-B radiation affects seedling emergence, DNA integrity, plant morphology, growth rate, and attractiveness to herbivore insects in Datura ferox
Solar ultraviolet-B radiation affects seedling emergence, DNA integrity, plant morphology, growth rate, and attractiveness to herbivore insects in Datura ferox
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DOI：
10.1104/pp.112.1.161
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发表时间：
1996-09-01
期刊：
影响因子：
7.4
通讯作者：
Yanovsky, MJ
中科院分区：
文献类型：
--
作者：
Ballare, CL;Scopel, AL;Yanovsky, MJ
为了研究太阳紫外线B辐射（UV-B）对野生植物生理的影响之间的函数关系，以夏季一年生曼陀罗（Datura ferox L.）为材料，进行了野外排除试验。布宜诺斯艾利斯上空的太阳紫外线B事件减少了白天的出苗，抑制了茎的伸长和叶的展开，并往往减少了早期生长期间的生物量积累。然而，UV-B对计算的净同化率没有影响。利用一种针对环丁烷-嘧啶二聚体（CPU）的单克隆抗体，我们发现，与受太阳UV-B屏蔽的植物相比，接受充足阳光照射的植物每单位DNA的CPD含量更高，但UV-B与CPD含量之间的正相关性在高UV-B水平（接近太阳）时趋于平稳。在我们的现场，曼陀罗植物被叶甲虫（鞘翅目）消耗，并受到昆虫攻击的植物的比例下降，在生长过程中收到的UV-B的量。田间试验表明，植物暴露于太阳紫外线B可使叶甲攻击的可能性减少一半。我们的研究结果突出了与缩放植物对太阳UV-B的响应相关的复杂性，因为它们表明：（a）UV-B对净同化率和整株植物生长率的影响之间缺乏对应关系，（B）非线性UV-B剂量响应曲线，（c）UV-B对植物吸引天然食草动物的影响。
To study functional relationships between the effects of solar ultraviolet-B radiation (UV-B) on different aspects of the physiology of a wild plant, we carried out exclusion experiments in the field with the summer annual Datura ferox L. Solar UV-B incident over Buenos Aires reduced daytime seedling emergence, inhibited stem elongation and leaf expansion, and tended to reduce biomass accumulation during early growth. However, UV-B had no effect on calculated net assimilation rate. Using a monoclonal antibody specific to the cyclobutane-pyrimidine dimer (CPU), we found that plants receiving full sunlight had more CPDs per unit of DNA than plants shielded from solar UV-B, but the positive correlation between UV-B and CPD burden tended to level oft at high (near solar) UV-B levels. At our field site, Datura plants were consumed by leaf beetles (Coleoptera), and the proportion of plants attacked by insects declined with the amount of UV-B received during growth. Field experiments showed that plant exposure to solar UV-B reduced the likelihood of leaf beetle attack by one-half. Our results highlight the complexities associated with scaling plant responses to solar UV-B, because they show: (a) a lack of correspondence between UV-B effects on net assimilation rate and whole-plant growth rate, (b) nonlinear UV-B dose-response curves, and (c) UV-B effects of plant attractiveness to natural herbivores.