Literature-Based Phenotype Survey and In Silico Genotype Investigation of Antibiotic Resistance in the GenusBifidobacterium
Literature-Based Phenotype Survey and In Silico Genotype Investigation of Antibiotic Resistance in the GenusBifidobacterium
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DOI：
10.1007/s00284-020-02230-w
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发表时间：
2020-10-14
影响因子：
2.6
通讯作者：
Yin, Yeshi
中科院分区：
文献类型：
--
作者：
Cao, Linyan;Chen, Huahai;Yin, Yeshi
双歧杆菌是典型的寄生于人体肠道的益生菌，由于其益生特性，对宿主有益。益生菌的风险之一是可能将抗生素抗性基因（ARG）引入宿主肠道病原体。本研究旨在通过结合已报道的表型数据集和计算机基因型预测来描述双歧杆菌属的一般抗生素耐药性特征。双歧杆菌对β-内酰胺类、糖肽类、氯霉素和利福平敏感，对氨基糖苷类、多肽类、喹诺酮类和莫匹罗星耐药。对红霉素、四环素、夫西地酸、甲硝唑、克林霉素和甲氧苄啶的耐药表型各不相同。除cmXandtetQ外，通过BLASTP搜索从831个双歧杆菌基因组中鉴定出3520个推定的ARG。鉴定的ARG与38个参考ARG匹配，其中4个似乎是突变的管家基因。两个高丰度的ARG，tetWandermX，被发现有不同的分布特征。预测的ARG合理地解释了已发表文献中大多数相应的耐药表型。
Bifidobacteria are typical commensals inhabiting the human intestine and are beneficial to the host because of their probiotic properties. One of the risks concerning probiotics is the potential of introducing antibiotic resistance genes (ARGs) to the host gut pathogens. This study was aimed to depict the general antibiotic resistance characteristics of the genusBifidobacteriumby combining the reported phenotype dataset and in silico genotype prediction. Bifidobacteria were mostly reported to be sensitive to beta-lactams, glycopeptides, chloramphenicol, and rifampicin, but resistant to aminoglycosides, polypeptides, quinolones, and mupirocin. Generally, the resistance phenotypes to erythromycin, tetracycline, fusidic acid, metronidazole, clindamycin, and trimethoprim were variable. BesidescmXandtetQ, characterized in bifidobacterial resident plasmids, 3520 putative ARGs were identified from 831 bifidobacterial genomes through BLASTP search. The identified ARGs matched thirty-eight reference ARGs, four of which seemed to be mutant housekeeping genes. The two high-abundant ARGs,tetWandermX, were found to have different distribution traits. The predicted ARGs reasonably explained most of the corresponding resistant phenotypes in the published literature.