Developmental basis of pronephric defects in Xenopus body plan phenotypes.
Developmental basis of pronephric defects in Xenopus body plan phenotypes.
复制标题
非洲爪蟾身体计划表型原肾缺陷的发育基础。
DOI：
10.1006/dbio.1999.9476
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Vize,PD
中科院分区：
文献类型：
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作者：
Seufert,DW;Brennan,HC;DeGuire,J;Jones,EA;Vize,PD
我们使用识别前肾小管或前肾管的单克隆抗体来探索非洲爪蟾胚胎发育中胚胎肾的诱导。在紫外线腹侧和锂背侧胚胎中检查了前肾的形态发生。我们发现前肾小管存在于除最强的紫外线诱导表型之外的所有表型中，但在相对中等的锂表型中不存在。有趣的是，从前肾原基的腹后部发展而来的前肾管，比前肾小管在更多的轻度紫外线表型中缺失。紫外线腹侧胚胎中形成前肾能力的丧失是由于能够诱导前肾中胚层的组织丧失而引起的，因为来自腹侧胚胎的边缘区外植体仍然能够对前肾诱导信号做出反应。外植体重组实验表明，负责紫外线腹侧胚胎中原发性丢失和正常发育过程中前发性诱导的组织是前体节。在相对温和的锂表型中，原肾的缺失具有与 UV 表型不同的发育基础，因为来自锂处理的胚胎的外植体是具有活性中胚层的重组体中原肾的有效诱导物，即使它们本身不单独形成原肾。这些数据共同表明，背侧组织，尤其是前体节，负责中间中胚层的建立和胚胎肾的诱导，即使是轻微的背侧化也会破坏形成能够接收该信号的细胞的能力。
We have used monoclonal antibodies that recognize the pronephric tubules or pronephric duct to explore the induction of the embryonic kidney in developing Xenopus embryos. Morphogenesis of the pronephros was examined in UV-ventralized and lithium-dorsalized embryos. We find that the pronephric tubules are present in all but the strongest UV-induced phenotypes, but absent from relatively moderate lithium phenotypes. Interestingly the pronephric duct, which develops from the ventroposterior portion of the pronephric anlage, is missing from more of the mild UV phenotypes than are pronephric tubules. The loss of the capacity to form pronephroi in UV-ventralized embryos is caused by the loss of tissues capable of inducing the pronephric mesoderm, as marginal zone explants from ventralized embryos are still competent to respond to pronephric-inductive signals. Explant recombination experiments indicate that the tissue responsible for both the loss of pronephroi in UV-ventralized embryos and the induction of pronephroi during normal development is the anterior somites. The absence of pronephroi in relatively mild lithium phenotypes has a developmental basis different from that of the UV phenotype, as explants from lithium-treated embryos are effective inducers of pronephroi in recombinants with competent mesoderm, even though they themselves do not form pronephroi in isolation. Together these data indicate that dorsal tissues, especially the anterior somites, are responsible for the establishment of the intermediate mesoderm and the induction of the embryonic kidneys and that even mild dorsalization destroys the capacity to form cells competent to receive this signal.