Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells
Comparison of cell membrane damage induced by the therapeutic ultrasound on human breast cancer MCF-7 and MCF-7/ADR cells
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超声治疗对人乳腺癌MCF-7和MCF-7/ADR细胞细胞膜损伤的比较
DOI：
10.1016/j.ultsonch.2015.03.001
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发表时间：
2015-09-01
影响因子：
8.4
通讯作者：
Wang, Xiaobing
中科院分区：
文献类型：
--
作者：
Jia, Yali;Yuan, Wenjuan;Wang, Xiaobing
目的：本研究的目的是比较不同强度超声对 MCF-7/ADR 细胞和 MCF-7 细胞引起的细胞膜损伤。方法：将培养皿（直径 35 mm）中的肿瘤细胞暴露于强度范围为 0.25 W/cm(2) 至 0.75 W/cm(2) 的平面超声下 60 s。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑(MU)测定和Guava Viacount测定测定细胞的活力。使用碘化丙啶 (PI) 染色和细胞摄取异硫氰酸荧光素-葡聚糖 (FD500)，通过流式细胞术评估细胞膜完整性。本文还利用分光光度法专门比较了两种细胞系的膜脂过氧化和膜流动性。扫描电镜观察膜表面超微结构的变化。结果：随着照射强度从0.25 W/cm(2)增加到0.75 W/cm(2)，超声对两种细胞系产生的细胞毒性均增强。超声应用后细胞膜通透性和脂质过氧化水平显着增强。此外，0.75 W/cm(2)超声处理后，扫描电镜下观察到相对严重的细胞损伤。结论：在相同实验条件下，超声暴露以强度依赖性方式降低MCF-7和MCF-7/ADR细胞活力，且MCF-7/ADR细胞比MCF-7细胞对超声暴露更敏感。 MCF-7/ADR细胞膜流动性下降可能是其膜损伤加剧的原因之一。 (C) 2015 Elsevier B.V. 保留所有权利。
Objectives: The aim of this study was to compare the cell membrane damage induced by ultrasound at different intensities between MCF-7/ADR cells and MCF-7 cells.Methods: Tumor cells in the culture dishes (35 mm diameter) were exposed to planner ultrasound at intensities range from 0.25 W/cm(2) to 0.75 W/cm(2) for 60 s. The viability of cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MU) assay and Guava Viacount assay. The cell membrane integrity was estimated by flow cytometry using propidium iodide (PI) staining and cellular uptake of fluorescein isothiocyanate-dextran (FD500). The membrane lipid peroxidation and membrane fluidity were also specially compared between two cell lines in this paper using spectrophotometry. Ultrastructural alterations on membrane surface were observed by scanning electron microscopy.Results: The ultrasound produced cytotoxicity in both cell lines increased with the irradiation intensity increased from 0.25 W/cm(2) to 0.75 W/cm(2). Cell membrane permeability and the level of lipid peroxidation were remarkably enhanced after ultrasound application. In addition, relatively severe cell damage was observed under scanning electron microscopy after 0.75 W/cm(2) ultrasound treatment.Conclusions: Ultrasound exposure decreased MCF-7 and MCF-7/ADR cell viability in an intensity-dependent manner and MCF-7/ADR cells were more sensitive to ultrasound exposure than MCF-7 cells at the same experimental conditions. The declined membrane fluidity in MCF-7/ADR cell may be one of the reasons for its increased membrane damage. (C) 2015 Elsevier B.V. All rights reserved.