Fungal inulinases: Enzymology, molecular biology and biotechnology
Fungal inulinases: Enzymology, molecular biology and biotechnology
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真菌菊粉酶：酶学、分子生物学和生物技术
DOI：
10.5458/jag.51.247
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发表时间：
2004
影响因子：
1.1
通讯作者：
S. Moriyama
中科院分区：
文献类型：
--
作者：
K. Ohta;H. Akimoto;S. Moriyama
菊糖是一种葡聚糖，其中β-（2→1）连接的d -葡聚糖分子的线性链在还原端与蔗糖相连。菊粉酶存在于高等植物和微生物中，包括丝状真菌、酵母和细菌。从丝状真菌中纯化出的外菊糖酶和内菊糖酶被定性为单体糖蛋白。从曲霉和青霉中克隆出菊粉酶基因，得到的氨基酸序列包含β-果糖呋喃苷酶超家族的保守序列。系统发育分析表明，真菌的外链酶和内链酶对菊粉的末端和内部β-(2→1)-果糖呋喃苷键具有各自独立的水解活性。将固定化形式的黑曲霉菊粉酶应用于菊粉连续生产果葡糖浆或菊粉低聚糖。以纯菊粉或菊芋块茎为原料，采用黑曲霉和酿酒酵母同时糖化和发酵的方法制备高浓度乙醇。
Inulin is a fructan in which linear chains of β-(2→1)-linked D-fructofuranose molecules are attached to sucrose at the reducing end. Inulinases have been encountered in higher plants and in microorganisms, including filamentous fungi, yeasts and bacteria. Exoinulinases exo- and endoinulinases from filamentous fungi have been purified and characterized as monomeric glycoproteins. The inulinase genes from Aspergillus and Penicillium spp. have been cloned and the deduced amino acid sequences included conserved sequences in the β-fructofuranosidase superfamily. A phylogenetic analysis showed that fungal exo- and endoinulinases have independently evolved the respective hydrolytic activities toward terminal and internal β-(2→1)-fructofuranosidic linkages in inulin. Aspergillus niger inulinases in an immobilized form were applied to the continuous production of fructose syrup or inulo-oligosaccharides from inulin. High concentrations of ethanol were produced from pure inulin or Jerusalem artichoke tubers by a simultaneous saccharification and fermentation process using A. niger and Saccharomyces cerevisiae.