SERUM-FREE MEDIUM FOR GENERATION AND PROPAGATION OF FUNCTIONAL HUMAN CYTO-TOXIC AND HELPER T-CELL CLONES
SERUM-FREE MEDIUM FOR GENERATION AND PROPAGATION OF FUNCTIONAL HUMAN CYTO-TOXIC AND HELPER T-CELL CLONES
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DOI：
10.1016/0022-1759(84)90450-2
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发表时间：
1984-01-01
影响因子：
2.2
通讯作者：
SPITS, H
中科院分区：
文献类型：
--
作者：
YSSEL, H;DEVRIES, JE;SPITS, H
描述了一种无血清淋巴细胞培养基，其中用牛血清白蛋白、转铁蛋白、胰岛素、乙醇胺以及饱和和不饱和脂肪酸（亚油酸、油酸和棕榈酸）的混合物代替血清。在这种无血清培养基中，混合淋巴细胞培养诱导的增殖和细胞毒性反应与含血清培养基中获得的反应相当。抗原特异性的细胞毒性和辅助性T细胞被分离出来，并且可以在无血清培养基中繁殖而不丧失功能。
A serum-free lymphocyte culture medium is described in which serum is replaced by bovine serum albumin, transferrin, insulin, ethanolamine, and a mixture of saturated and unsaturated fatty acids (linoleic acid, oleic acid and palmitic acid). In this serum-free medium proliferative and cytotoxic responses induced in mixed lymphocyte culture were comparable with those obtained in medium containing serum. Antigen-specific cytotoxic and helper T cells were isolated and could be propagated in serum-free medium without loss of function.