Structure and antiviral activity of the galactofucan sulfates extracted from Undaria Pinnatifida (Phaeophyta)
Structure and antiviral activity of the galactofucan sulfates extracted from Undaria Pinnatifida (Phaeophyta)
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DOI：
10.1007/s10811-006-9096-9
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发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Thompson, K.
中科院分区：
文献类型：
--
作者：
Hemmingson, J. A.;Falshaw, R.;Thompson, K.
通过稀酸提取从褐藻裙带菜中获得的硫酸半乳岩藻聚糖提取物(GFS)是疱疹病毒HSV-1、HSV-2和HCMV的有效抑制剂，体外测定的IC(50)值分别为1.1、0.2和0.5μgmL(-1)。通过阴离子交换色谱法对 GFS 进行分级分离，得到三个级分，其糖醛酸和硫酸盐含量及其抗病毒活性不同，并且与 GFS 相比分子量略有降低。低糖醛酸/高硫酸盐级分 (F2M) 的产率为 63%，具有相似的吡喃半乳糖基和吡喃岩藻糖基残基摩尔比例，几乎没有相关蛋白质，并且与 GFS 等价（IC(50) 值分别为 1.1、0.1 和 0.5 mu gmL(-1)）。高糖醛酸/低硫酸盐级分(F1M)的产率为18%，其吡喃半乳糖基残基比例低得多，且活性较低(IC(50)值分别为4.6、1.0和4.0μgmL(-1))。次要的低糖醛酸/高硫酸盐组分（F4M）具有显着量的相关蛋白并且活性也较低（IC（50）分别= 3.1、1.0和2.0μgmL（-1））。通过溶剂解脱硫前后的糖基连接分析，显示主要部分 (F2M) 的结构很复杂，并且鉴定出许多成分糖残基，尽管 3-连接的吡喃岩藻糖基 2,4-二硫酸残基是一个突出特征。
The galactofucan sulfate extract (GFS) obtained from the brown seaweed Undaria pinnatifida by extraction with dilute acid is a potent inhibitor of the herpes viruses HSV-1, HSV-2 and HCMV, with IC(50) values determined in vitro of 1.1, 0.2 and 0.5 mu gmL(-1), respectively. Fractionation of GFS by anion exchange chromatography gave three fractions which differed in their uronic acid and sulfate contents and in their antiviral activity, as well as in having somewhat reduced molecular weights compared to GFS. The low uronic acid/high sulfate fraction (F2M), obtained in 63% yield, had similar molar proportions of galactopyranosyl and fucopyranosyl residues, little associated protein and was equipotent with GFS (IC(50) values of 1.1, 0.1 and 0.5 mu gmL(-1), respectively). The high uronic acid/low sulfate fraction (F1M), obtained in 18% yield, had a much lower proportion of galactopyranosyl residues and was less active (IC(50) values of 4.6, 1.0 and 4.0 mu gmL(-1), respectively). The minor low uronic acid/high sulfate fraction (F4M) had a significant amount of associated protein and was also less active (IC(50) = 3.1, 1.0 and 2.0 mu gmL(-1), respectively). The structure of the major fraction (F2M) was shown to be complex by glycosyl linkage analysis before and after solvolytic desulfation, with many component sugar residues being identified, although 3-linked fucopyranosyl 2,4-disulfate residues were a prominent feature.