LUNG MYELOPEROXIDASE AS A MEASURE OF PULMONARY LEUKOSTASIS IN RABBITS
LUNG MYELOPEROXIDASE AS A MEASURE OF PULMONARY LEUKOSTASIS IN RABBITS
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DOI：
10.1152/jappl.1985.59.6.1978
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发表时间：
1985-01-01
影响因子：
3.3
通讯作者：
JAY, M
中科院分区：
文献类型：
--
作者：
GOLDBLUM, SE;WU, KM;JAY, M
肺白细胞淤滞可能与急性肺损伤有关。我们用髓过氧化物酶（MPO）作为粒细胞标记物来定量兔肺白细胞淤积，研究了肺过氧化物酶活性。将肺在洗涤剂中均质化，冻融，超声处理，离心，并测定上清液的MPO。进行了7次提取，在前3次提取中发现累积MPO> 80%。通过使用三次提取程序，平均肺MPO（Δ A）min-1. g组织-1）在正常[20.9 . ±. 5.2（SE）]、粒细胞耗竭（6.5 ±. 2.0）、盐水注射（22.2 . ±. 5.6）和肺炎球菌（PNC）攻击的（69.7 . ±. 10.6）动物。与正常动物相比，粒细胞耗竭组肺MPO显著降低（P < 0.005），而与盐水注射组相比，PNC攻击组肺MPO显著升高（P < 0.001）。从粒细胞和肺中提取的MPO来自正常以及PNC攻击的动物都是生化相同的。肺提取物不抑制MPO，从白细胞抑制肺获得的支气管肺泡灌洗液中未检测到MPO。肺组织MPO与肺血管内粒细胞计数呈显著正相关（P < 0.01）。肺MPO测定是一种相对简单的定量方法，可用于检测肺白细胞淤积。
Pulmonary leukostasis can be associated with acute lung injury. We studied lung peroxidase activity using myeloperoxidase (MPO) as a granulocyte marker to quantitate pulmonary leukostasis in rabbits. Lungs were homogenized in detergent, freeze-thawed, sonified, and centrifuged, and supernatants were assayed for MPO. Seven extractions were performed, and > 80% of cumulative MPO was found in the first three extractions. By use of a three-extraction procedure, the mean lung MPO (.DELTA.A .cntdot. min-1 .cntdot. g tissue-1) was determined in normal [20.9 .+-. 5.2 (SE)], granulocyte-depleted (6.5 .+-. 2.0), saline-injected (22.2 .+-. 5.6), and pneumococcus (PNC)-challenged (69.7 .+-. 10.6) animals. Lung MPO was significantly decreased in granulocyte-depleted compared with normal animals (P < 0.005) and significantly increased in PNC-challenged compared with saline-injected animals (P < 0.001). MPO extracted from granulocytes and lungs from normal as well as PNC-challenged animals were all biochemically identical. Lung extract did not inhibit MPO, and no MPO was detected in bronchoalveolar lavage fluid obtained from leukostatic lungs. Lung MPO significantly (P < 0.01) correlated with intravascular intrapulmonary granulocytes. Determination of lung MPO is a relatively simple quantitative method that can be used to detect pulmonary leukostasis.