Zingiber officinale Roscoe alone and in combination with α-tocopherol protect the kidney against cisplatin-induced acute renal failure
Zingiber officinale Roscoe alone and in combination with α-tocopherol protect the kidney against cisplatin-induced acute renal failure
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DOI：
10.1016/j.fct.2006.11.014
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发表时间：
2007-06-01
影响因子：
4.3
通讯作者：
Usha, S.
中科院分区：
文献类型：
--
作者：
Ajith, T. A.;Nivitha, V.;Usha, S.
由于活性氧分子（ROM）的异常产生而引起的氧化应激被认为与许多外源性物质毒性的病因学有关。有证据表明，ROM参与了广泛使用的合成抗癌药物顺铂的肾毒性。使用顺铂（10 mg/kg体重的单剂量，i.p）诱导的小鼠急性肾损伤，评价了单独的和与维生素E（α-生育酚）组合的石斛乙醇提取物的肾保护作用。实验结果表明，中药两面针对顺铂所致的肾毒性有明显的保护作用，并呈剂量依赖性。顺铂单独治疗组的血清尿素和肌酐水平相对于正常组动物显著升高（P < 0.01）。在Z中的水平降低。officinale（250和500 mg/kg，p.o.）+顺铂，维生素E（250 mg/kg，p.o.）+顺铂;维生素E+维生素E治疗组。单用顺铂组大鼠肾脏抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）活性及还原型谷胱甘肽（GSH）水平下降，丙二醛（MDA）水平升高。与对照组相比，Z组SOD、CAT、GPx活性和GSH含量显著升高，MDA含量显著降低（P < 0.05）。officinale（250和500 mg/kg）+顺铂和Z.维生素E+顺铂治疗组（250 mg/kg）。试验结果表明，药用植物Z（250 mg/kg体重）的保护效果优于维生素E（250 mg/ kg体重）。结果还表明，Z.与维生素E（250 mg/kg）一起使用的药用植物（250 mg/kg）与其250 mg/kg单独处理组相比显示出更好的保护作用。本研究结果表明，Z.单独使用和与维生素E联合使用可部分改善顺铂诱导的肾毒性。这种保护作用是通过阻止顺铂诱导的肾脏抗氧化防御系统的下降或通过其直接的自由基清除活性介导的。(C)2006爱思唯尔有限公司保留所有权利。
Oxidative stress due to abnormal production of reactive oxygen molecules (ROM) is believed to be involved in the etiology of toxicities of many xenobiotics. Evidences suggested that ROM is involved in the nephrotoxicity of a widely used synthetic anticancer drug cisplatin. The nephroprotective effects of ethanol extract of Zingiber officinale alone and in combination with vitamin E (alpha-tocopherol) were evaluated using cisplatin (single dose of 10 mg/kg body wt, i.p) induced acute renal damage in mice. The results of the study indicated that Z officinale significantly and dose dependently protected the nephrotoxicity induced by cisplatin. The serum urea and creatinine levels in the cisplatin alone treated group were significantly elevated (P < 0.01) with respect to normal group of animals. The levels were reduced in the Z. officinale (250 and 500 mg/kg, p.o) plus cisplatin, vitamin E (250 mg/kg) plus cisplatin, and Z. officinale (250 mg/kg) with vitamin E plus vitamin E treated groups. The renal antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities and level of reduced glutathione (GSH) were declined; level of malondialdehyde (MDA) was elevated in the cisplatin alone treated group. The activities of SOD, CAT GPx and level of GSH were elevated and level of MDA declined significantly (P < 0.05) in the Z. officinale (250 and 500 mg/kg) plus cisplatin and Z. officinale (250 mg/kg) with vitamin E plus cisplatin treated groups. The protective effect of Z officinale (250 mg/kg body wt) was found to be better than that of vitamin E (250 mg/ kg body wt). The results also demonstrated that combination of Z. officinale (250 mg/kg) with vitamin E (250 mg/kg) showed a better protection compared to their 250 mg/kg alone treated groups. This study concluded that ethanol extract of Z. officinale alone and in combination with vitamin E partially ameliorated cisplatin-induced nephrotoxicity. This protection is mediated either by preventing the cisplatin-induced decline of renal antioxidant defense system or by their direct free radical scavenging activity. (C) 2006 Elsevier Ltd. All rights reserved.