Confocal microscopy of the light organ crypts in juvenile Euprymna scolopes reveals their morphological complexity and dynamic function in symbiosis.
Confocal microscopy of the light organ crypts in juvenile Euprymna scolopes reveals their morphological complexity and dynamic function in symbiosis.
复制标题
幼年 Euprymna scolopes 光器官隐窝的共聚焦显微镜揭示了它们的形态复杂性和共生中的动态功能。
DOI：
10.1002/jmor.10422
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
McFall-Ngai,Margaret
中科院分区：
文献类型：
--
作者：
Sycuro,LauraK;Ruby,EdwardG;McFall-Ngai,Margaret
In the hours to days following hatching, the Hawaiian bobtail squid,Euprymna scolopes,obtains its light‐emitting symbiont,Vibrio fischeri, from the surrounding environment and propagates the bacteria in the epithelial crypts of a specialized light organ. Three‐dimensional analyses using confocal microscopy revealed that each of the three crypts on either side of the juvenile light organ is composed of four morphological regions. Progressing from the lateral pore to the medial blind end of each crypt, the regions consist of 1) a duct, 2) an antechamber, 3) a bottleneck, and 4) a deep region. Only the deep region houses a persistent bacterial population, whereas the duct, antechamber, and bottleneck serve as conduits through which the bacteria enter during initial colonization and exit during diel venting, a behavior in which ∼90% of the symbionts are expelled each dawn. Our data suggest that, like the duct, the antechamber and bottleneck may function to promote and maintain the specificity of the symbiosis. Pronounced structural and functional differences among the deep regions of the three crypts, along with previously reported characterizations of embryogenesis, suggest a continued developmental progression in the first few days after hatching. Taken together, the results of this study reveal a high degree of complexity in the morphology of the crypts, as well as in the extent to which the three crypts and their constituent regions differ in function during the early stages of the symbiosis. J. Morphol. © 2006 Wiley‐Liss, Inc.
