Archaeal diversity along a soil salinity gradient prone to disturbance.
Archaeal diversity along a soil salinity gradient prone to disturbance.
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DOI：
10.1111/j.1462-2920.2005.00864.x
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发表时间：
2005-10
影响因子：
5.1
通讯作者：
D. Walsh;R. Papke;W. Doolittle
中科院分区：
文献类型：
--
作者：
D. Walsh;R. Papke;W. Doolittle
我们采用了一种独立于种植的方法来研究加拿大不列颠哥伦比亚省盐泉沿瞬态土壤盐度梯度的古菌多样性，该盐泉经常因强降雨而受到侵蚀。使用从沿该梯度收集的三个土壤样本中提取的 DNA 创建古菌 16S rRNA 基因库。统计比较表明，不同地点的古菌丰富度相似，但古菌群落组成沿盐度梯度发生显着变化。土壤文库中有七个不同的系统发育群体。 Haloarchaea 是最常采样的群体。其他 16S rRNA 序列与未培养的广古菌门和泉古菌门或嗜盐产甲烷菌有关。与之前表征的盐古菌群落相比，盐度较高的土壤中的盐古菌多样性非常高。盐泉盐古菌与已知的低盐适应/耐受物种关系并不密切，这表明它们可能经常因土壤盐度频繁下降而面临局部死亡。我们推测，以盐度波动的形式出现的生态系统干扰是维持盐泉盐古菌多样性群落的一种机制。
We employed a cultivation-independent approach to examine archaeal diversity along a transient soil salinity gradient at Salt Spring in British Columbia, Canada that is routinely eroded due to heavy, recurrent rainfall. Archaeal 16S rRNA gene libraries were created using DNA extracted from three soil samples collected along this gradient. Statistical comparisons indicated similar archaeal richness across sites but, a significant shift in archaeal community composition along the salinity gradient. Seven distinct phylogenetic groups were represented in soil libraries. Haloarchaea were the most commonly sampled group. Other 16S rRNA sequences were related to uncultured Euryarchaeota and Crenarchaeota or halophilic methanogens. Haloarchaeal diversity was remarkably high in soil of elevated salinity compared with previously characterized haloarchaeal communities. Salt Spring haloarchaea were not closely related to known low-salt adapted/tolerant species, suggesting they may be frequently faced with local mortality as a result of frequent declines in soil salinity. We speculate that ecosystem disturbance -- in the form of salinity fluctuations -- is one mechanism for maintaining a diverse community of haloarchaea at Salt Spring.