Changes in permeability of dog tracheal epithelium in response to hydrostatic pressure.
Changes in permeability of dog tracheal epithelium in response to hydrostatic pressure.
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狗气管上皮的渗透性随静水压力的变化。
DOI：
10.1152/ajplung.1992.262.2.l176
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Widdicombe,JH
中科院分区：
文献类型：
--
作者：
Kondo,M;Finkbeiner,WE;Widdicombe,JH
我们验证了这样一个假设，即哮喘中气道上皮损伤和血蛋白渗漏到管腔中是水肿和粘膜下静水压力升高的结果。将犬气管上皮片固定在Ussing室中，并测定跨上皮流体静压差（Δ P）对电导（G）、[3 H]甘露醇通量（Jman）和异硫氰酸荧光素-白蛋白通量（Jalb）的影响。从组织的粘膜侧到粘膜下侧（m-s）的20 cmH 2 O的Δ P值对G、Jman、Jalb或组织超微结构没有显著影响。Δ Ps-;m引起电导（G）的增加，在约20 cmH 2 O处具有最大效应。Δ Ps-20 cmH 2 O的m显著（P <0.05）增加G（4.3 +/- 0.6至10.6 +/- 1.6 mS/cm 2），Jman s-m（18 +/- 5至411 +/- 54 nmol.cm-2.h-1）、J（alb）s-m（0.3 +/- 0.1至6.0 +/- 2.0微克.cm-2.h-1）和J（alb）m-s（0.7 +/- 0.3至1.8 +/- 0.4微克.cm-2.h-1）。Jman m-s没有受到影响。去除δ P后，G和Jman s-m恢复到暴露前值，但J（alb）s-m仍略高于1.1 +/- 0.3微克·cm-2·h-1。形态学上，三角洲Ps-m引起扩张的横向细胞间隙，破坏紧密连接，和粘膜下水肿。白蛋白和甘露醇的s-m通量的大幅增加与通过上皮损伤区域流向管腔的大量液体一致。
We tested the hypothesis that, in asthma, the airway epithelial damage and leakage of blood proteins into the lumen are the result of edema and raised submucosal hydrostatic pressure. Sheets of dog tracheal epithelium were mounted in Ussing chambers, and the effects of transepithelial hydrostatic pressure differences (delta P) on conductance (G), [3H]mannitol flux (Jman), and fluorescein isothiocyanate-albumin flux (Jalb) were determined. delta P values of 20 cmH2O directed from the mucosal to submucosal side of the tissue (m----s) had no significant effects on G, Jman, Jalb, or tissue ultrastructure. delta Ps----;m caused increases in conductance (G) with a maximal effect at approximately 20 cmH2O. delta Ps----m of 20 cmH2O significantly (P less than 0.05) increased G (4.3 +/- 0.6 to 10.6 +/- 1.6 mS/cm2), Jman s—m (18 +/- 5 to 411 +/- 54 nmol.cm-2.h-1), J(alb)s----m (0.3 +/- 0.1 to 6.0 +/- 2.0 micrograms.cm-2.h-1), and J(alb)m—s (0.7 +/- 0.3 to 1.8 +/- 0.4 micrograms.cm-2.h-1). Jman m----s was not affected. On removal of delta P, G and Jman s----m returned to preexposure values, though J(alb)s----m remained slightly elevated at 1.1 +/- 0.3 micrograms.cm-2.h-1. Morphologically, delta Ps----m caused dilation of lateral intercellular spaces, disruption of tight junctions, and submucosal edema. The large increases in s----m fluxes of albumin and mannitol are consistent with bulk flow of fluid toward the lumen via the areas of epithelial damage.