The effects of insulin on myocardial metabolism and acidosis in normoxia and ischaemia. A 31P-NMR study.
The effects of insulin on myocardial metabolism and acidosis in normoxia and ischaemia. A 31P-NMR study.
复制标题
常氧和缺血状态下胰岛素对心肌代谢和酸中毒的影响。
DOI：
10.1016/0167-4889(82)90034-9
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Williams,SR
中科院分区：
文献类型：
--
作者：
Bailey,IA;Radda,GK;Seymour,AM;Williams,SR
1.用~（31）P-NMR研究了胰岛素对常氧和完全缺血大鼠心脏的作用。2.在常氧灌注，胰岛素增加磷酸肌酸ATP的比例在牺牲Pi，当葡萄糖是底物。当乙酸盐用作唯一底物时，未观察到变化。细胞内pH值（从2-脱氧葡萄糖6-磷酸共振峰的位置测量）不受胰岛素治疗的影响。3.缺血前输注胰岛素导致无血流期间酸中毒的速率和程度增加，而ATP耗竭速率降低。4.冷冻夹闭研究表明，葡萄糖灌注大鼠心脏的胰岛素治疗后，糖原水平增加。在缺血期间，观察到糖原含量减少，同时乳酸增加。胰岛素治疗的结果是缺血期间糖原对磷酸化酶的可及性增加。在缺血期间的糖酵解的控制进行了讨论，相对于在心脏组织中的糖原的含量和结构。
1. The effect of insulin on the perfused rat heart during normoxia and total ischaemia was studied by31P-NMR. 2. During normoxic perfusion, insulin increased the phosphocreatine to ATP ratio at the expense of Pi, when glucose was the substrate. No change was observed when acetate was used as the sole substrate. The intracellular pH (as measured from the position of the 2-deoxyglucose 6-phosphate resonance peak) was unaffected by insulin treatment. 3. Infusion of insulin prior to ischaemia caused an increase in the rate and extent of acidosis during the period of no flow while the rate of ATP depletion was decreased. 4. Freezeclamped studies showed an increase in glycogen levels upon insulin treatment of the glucose perfused rat heart. During ischaemia, a decrease in glycogen content concomitant with an increase in lactate was observed. The accessibility of glycogen to phosphorylase during ischaemia is increased as a result of insulin treatment. The control of glycolysis during ischaemia is discussed with respect to the content and structure of glycogen in heart tissue.