Design and characterization of antitumor drug paclitaxel-loaded chitosan nanoparticles by W/O emulsions
Design and characterization of antitumor drug paclitaxel-loaded chitosan nanoparticles by W/O emulsions
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DOI：
10.1016/j.ijbiomac.2011.12.034
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发表时间：
2012-03-01
影响因子：
8.2
通讯作者：
Ma, Guiping
中科院分区：
文献类型：
--
作者：
Xu, Juan;Ma, Lili;Ma, Guiping
以戊二醛为交联剂，在W/O乳化体系中，采用乳化-交联法制备了壳聚糖纳米粒和紫杉醇壳聚糖纳米粒。壳聚糖纳米粒的平均粒径随反应体系pH值从4.5增加到6.5而减小，当pH超过6.5时增大。紫外光谱分析表明，最大载药率为8.55%，包封率为94.01%。采用紫外分光光度法测定其体外释药曲线。MU分析表明，空白壳聚糖纳米粒几乎没有毒性，并进行了细胞培养。(C)2012爱思唯尔B.V.保留所有权利。
Chitosan nanoparticles and paclitaxel loaded chitosan nanoparticles were prepared by emulsification-crosslinking method in a W/O emulsion system, using glutaraldehyde as crosslinking agent. The mean diameter of chitosan nanoparticles decreased with increase of pH value of the reaction system from 4.5 to 6.5, and increased when the pH exceeded 6.5. Ultraviolet spectrum analysis showed that the largest loading efficiency and encapsulation efficiency could be 8.55% and 94.01%, respectively. In vitro drug release profile was also determined by ultraviolet spectrometry. MU assays revealed that the blank chitosan nanoparticles had almost none toxicity, and cell culture was carried out accordingly. (C) 2012 Elsevier B.V. All rights reserved.