Correlation of a unique 220-kDa protein with vitamin D sensitivity in glioma cells.
Correlation of a unique 220-kDa protein with vitamin D sensitivity in glioma cells.
复制标题
神经胶质瘤细胞中独特的 220 kDa 蛋白质与维生素 D 敏感性的相关性。
DOI：
10.1016/s0006-2952(00)00438-x
复制
发表时间：
2000
影响因子：
5.8
通讯作者：
Savaraj,N
中科院分区：
文献类型：
--
作者：
Zou,J;Landy,H;Feun,L;Xu,R;Lampidis,T;Wu,CJ;Furst,AJ;Savaraj,N
我们研究了1，25-二羟维生素D3（VD 3）在胶质瘤细胞系和来自患者手术标本的原代培养物中的抗肿瘤和凋亡作用。我们的研究结果表明，某些胶质瘤细胞发生凋亡，而其他的耐药。在试图寻找决定VD 3敏感性的参数时，我们在胶质瘤细胞中发现了一种对VD 3敏感的独特的220-kDa蛋白。该蛋白不是经典的维生素D受体（VDR），但被两种不同的抗VDR单克隆抗体识别。此外，在这些胶质瘤细胞中，220-kDa蛋白的水平与VD 3的ic 50呈负相关。这种220 kDa的蛋白质也存在于冷冻的脑肿瘤样品中，并且表达水平似乎与其相应的原代培养物相关。因此，我们的研究结果表明，这个220 kDa的蛋白质可能在决定恶性胶质瘤的VD 3敏感性中发挥重要作用。
We have investigated the antitumor and apoptotic effects of 1,25-dihydroxyvitamin D3(VD3) in glioma cell lines and in primary cultures derived from surgical specimens from patients. Our results showed that certain glioma cells underwent apoptosis, whereas others were resistant. In an attempt to search for parameters that dictate VD3sensitivity, we discovered a unique 220-kDa protein in glioma cells that were sensitive to VD3. This protein was not a classical vitamin D receptor (VDR), but was recognized by two different anti-VDR monoclonal antibodies. Furthermore, the level of the 220-kDa protein was inversely correlated with the ic50of VD3in these glioma cells. This 220-kDa protein was also present in frozen brain tumor samples, and the level of expression appeared to correlate with their corresponding primary cultures. Thus, our findings suggest that this 220-kDa protein may play an important role in determining VD3sensitivity in malignant glioma.