Heat-Shock Proteins MoSsb1, MoSsz1, and MoZuo1 Attenuate MoMkk1-Mediated Cell-Wall Integrity Signaling and Are Important for Growth and Pathogenicity of Magnaporthe oryzae.
Heat-Shock Proteins MoSsb1, MoSsz1, and MoZuo1 Attenuate MoMkk1-Mediated Cell-Wall Integrity Signaling and Are Important for Growth and Pathogenicity of Magnaporthe oryzae.
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DOI：
10.1094/mpmi-02-18-0052-r
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Zhang Z
中科院分区：
文献类型：
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作者：
Yang J;Liu M;Liu X;Yin Z;Sun Y;Zhang H;Zheng X;Wang P;Zhang Z
有丝分裂原活化蛋白激酶(MAPK)MoMkk1调控稻瘟病菌细胞壁完整性(CWI)途径。为了了解其潜在的机制，我们将MoSsb1确定为MoMkk1相互作用蛋白之一。MoSsb1是一个应激70亚家族B(SSB)蛋白同源物，与70 kDa热休克蛋白(Hsp70)有很高的氨基酸序列同源性。HSP70是一个保守的、普遍表达的伴侣蛋白家族，通过促进蛋白质折叠、防止蛋白质聚集和控制蛋白质降解来调节蛋白质的生物发生。我们发现MoSsb1调节新生多肽链的合成，这种调节是通过与Hsp70s MoSsz1和40-kDa Hsp40MoZuo1的其他成员形成复合体来实现的。MoSsb1对稻瘟病菌的生长、分生和完全致病力具有重要作用，MoSsz1和MoZuo1也具有同样的作用。重要的是，MoSsb1、MoSsz1和MoZuo1都通过调节MoMkk1的生物合成参与了CWI MAPK通路的调节。我们的研究揭示了MoSsb1、MoSsz1和MoZuo1如何影响CWI信号，参与调控米曲霉的生长、分化和毒力，并强调了热休克蛋白在病原真菌中的保守作用机制。
The mitogen-activated protein kinase (MAPK) MoMkk1 governs the cell-wall integrity (CWI) pathway in rice blast fungus Magnaporthe oryzae. To understand the underlying mechanism, we have identified MoSsb1 as one of the MoMkk1-interacting proteins. MoSsb1 is a stress-seventy subfamily B (Ssb) protein homolog, sharing high amino acid sequence homology with the 70-kDa heat shock proteins (Hsp70s). Hsp70 are a family of conserved and ubiquitously expressed chaperones that regulate protein biogenesis by promoting protein folding, preventing protein aggregation, and controlling protein degradation. We found that MoSsb1 regulates the synthesis of nascent polypeptide chains and this regulation is achieved by being in complex with other members of Hsp70s MoSsz1 and 40-kDa Hsp40 MoZuo1. MoSsb1 is important for the growth, conidiation, and full virulence of the blast fungus and this role is also shared by MoSsz1 and MoZuo1. Importantly, MoSsb1, MoSsz1, and MoZuo1 are all involved in the regulation of the CWI MAPK pathway by modulating MoMkk1 biosynthesis. Our studies reveal novel insights into how MoSsb1, MoSsz1, and MoZuo1 affect CWI signaling that is involved in regulating growth, differentiation, and virulence of M. oryzae and highlight the conserved functional mechanisms of heat-shock proteins in pathogenic fungi.