MEROSIN PROMOTES NEURITE GROWTH AND SCHWANN-CELL MIGRATION IN-VITRO AND NERVE REGENERATION IN-VIVO - EVIDENCE USING AN ANTIBODY TO MEROSIN, ARM-1
MEROSIN PROMOTES NEURITE GROWTH AND SCHWANN-CELL MIGRATION IN-VITRO AND NERVE REGENERATION IN-VIVO - EVIDENCE USING AN ANTIBODY TO MEROSIN, ARM-1
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DOI：
10.1006/dbio.1994.1186
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发表时间：
1994-07-01
影响因子：
2.7
通讯作者：
MATTHEW, WD
中科院分区：
文献类型：
--
作者：
ANTON, ES;SANDROCK, AW;MATTHEW, WD
阻断特异性抗原的细胞功能的单克隆抗体可以为体外和体内生物测定提供有价值的探针。为了了解发育和再生过程中神经元-雪旺细胞相互作用的分子基础，我们寻找了干扰雪旺细胞基底膜中存在的主要层粘连蛋白亚型--裂球蛋白功能的抗体。为了确定周围神经系统中的merosin的生物学功能，我们研究了许旺细胞迁移和神经突起生长在体外和神经再生在体内，在存在和不存在的单克隆抗体，我们认为结合到merosin，ARM-1（抗啮齿动物Merosin-1）。ARM-1抗体在体外阻断神经突生长和雪旺细胞迁移。该抗体还在体外减少神经炎分支。在体内，ARM-1抗体阻断了大鼠虹膜中交感神经纤维的再生。ARM-1抗体对神经突生长和雪旺细胞迁移的阻断表明，在周围神经的正常发育和再生过程中，merosin参与促进两个最关键的细胞事件，即，轴突生长和雪旺细胞迁移。此外，ARM-1抗原对雪旺细胞迁移和轴突生长的调节意味着这两种细胞事件在外周神经的发育和再生期间服从一组共同的分子信号。(C)1994年出版社出版。
Monoclonal antibodies that block the cellular function(s) of specific antigens can provide valuable probes for in vitro and in vivo bioassays. With the goal of understanding the molecular basis of neuron-Schwann cell interactions during development and regeneration, we have sought antibodies that interfere with the function of merosin, the predominant laminin isoform present in the Schwann cell basement membrane. To identify the biological functions of merosin in the peripheral nervous system, we studied Schwann cell migration and neurite outgrowth in vitro and nerve regeneration in vivo, in the presence and the absence of a monoclonal antibody that we believe binds to merosin, ARM-1 (Anti Rodent Merosin-1). The ARM-1 antibody blocked both neurite outgrowth and Schwann cell migration in vitro. This antibody also reduced neuritic branching in vitro. In vivo, the ARM-1 antibody blocked the regeneration of sympathetic nerve fibers in the rat iris. The blockade of neurite outgrowth and Schwann cell migration by ARM-1 antibody suggests that merosin is involved in facilitating the two most critical cellular events during the normal development and regeneration of peripheral nerves, i.e., axon growth and Schwann cell migration. Furthermore, the regulation of both Schwann cell migration and axon growth by the ARM-1 antigen implies that these two cellular events obey a common set of molecular signals during the development and regeneration of peripheral nerves. (C) 1994 Academic Press, Inc.