An extensive study of human IgE cross-reactivity of Blo t 5 and Der p 5
An extensive study of human IgE cross-reactivity of Blo t 5 and Der p 5
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DOI：
10.1067/mai.2003.167
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发表时间：
2003-03-01
影响因子：
14.2
通讯作者：
Chua, KY
中科院分区：
文献类型：
--
作者：
Kuo, IC;Cheong, N;Chua, KY
Dual sensitization by Blomia tropicalis and Dermatophagoides pteronyssinus mites is common in tropical and subtropical countries. The human IgE cross-reactivity between clinical important group 5 allergens, Blo t 5 and Der p 5, remains controversial.Objective: This study was undertaken to assess the levels of the IgE cross-reactivity between Blo t 5 and Der p 5 by using sera from a large cohort of asthmatic children in subtropical and tropical countries.Methods: Purified recombinant Blo 15 and Der p 5 were produced in Pichia pastoris and tested against sera from 195 asthmatic children. The IgE cross-reactivity was examined by direct, inhibitory and competitive human IgE enzyme-linked immunosorbent assay as well as skin prick tests.Results: The Blo t 5 IgE responses were 91.8% (134 of 146) and 73.5% (36 of 49) for Taiwanese and Malaysian sera, respectively. The Blo t 5 specific IgE titers were significantly higher than those of Der p 5 (P
