Variations on a theme: evolution of the phage-shock-protein system in Actinobacteria.
Variations on a theme: evolution of the phage-shock-protein system in Actinobacteria.
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主题的变体：放线菌中噬菌体休克蛋白系统的进化。
DOI：
10.1007/s10482-018-1053-5
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Gennaro,MariaLaura
中科院分区：
文献类型：
--
作者：
Ravi,Janani;Anantharaman,Vivek;Aravind,L;Gennaro,MariaLaura
The phage shock protein (Psp) stress-response system protects bacteria from envelope stress through a cascade of interactions with other proteins and membrane lipids to stabilize the cell membrane. A key component of this multi-gene system is PspA, an effector protein that is found in diverse bacterial phyla, archaea, cyanobacteria, and chloroplasts. Other members of the Psp system include the cognate partners of PspA that are part of known operons:pspF||pspABCin Proteobacteria,liaIHGFSRin Firmicutes, andclgRpspAMNin Actinobacteria. Despite the functional significance of the Psp system, the conservation of PspA and other Psp functions, as well as the various genomic contexts of PspA, remain poorly characterized in Actinobacteria. Here we utilize a computational evolutionary approach to systematically identify the variations of the Psp system in ~450 completed actinobacterial genomes. We first determined the homologs of PspA and its cognate partners (as reported inEscherichia coli,Bacillus subtilis, andMycobacterium tuberculosis) across Actinobacteria. This survey revealed that PspA and most of its functional partners are prevalent in Actinobacteria. We then found that PspA occurs in four predominant genomic contexts within Actinobacteria, the primary context being theclgRpspAMsystem previously identified in Mycobacteria. We also constructed a phylogenetic tree of PspA homologs (including paralogs) to trace the conservation and evolution of PspA across Actinobacteria. The genomic context revealed that PspA shows changes in its gene-neighborhood. The presence of multiple PspA contexts or of other known Psp members in genomic neighborhoods that do not carrypspAsuggests yet undiscovered functional implications in envelope stress response mechanisms.
