Differentiation between pyometra and cystic endometrial hyperplasia/mucometra in bitches by prostaglandin F2α metabolite analysis
Differentiation between pyometra and cystic endometrial hyperplasia/mucometra in bitches by prostaglandin F2α metabolite analysis
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DOI：
10.1016/j.theriogenology.2005.11.002
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发表时间：
2006-07-15
期刊：
影响因子：
2.8
通讯作者：
Lagerstedt, A. -S.
中科院分区：
文献类型：
--
作者：
Hagman, R.;Kindahl, H.;Lagerstedt, A. -S.
脓膜增生的母狗是潜在的急诊病例，可能在临床上难以与囊性子宫内膜增生（CEH）合并粘膜肿大的病例区分。本研究测定59只脓脓母狗、10只CEH母狗和9只对照母狗血浆中前列腺素F-2 α的主要代谢物15-酮-13,14-二氢- pgf (2 α) （pg代谢物）浓度、血液生化和血液学参数，以确定pg代谢物是否能区分三种子宫状况。脓脓病母狗血浆pg代谢物水平显著高于CEH母狗（P = 0.002）和对照组（P = 0.002）。在诊断为子宫积液的母狗中，单独的pg代谢物分析对脓膜瘤与CEH的鉴别具有高灵敏度（98.3%）和高特异性（80.0%）。当结合pg代谢物和百分比带中性粒细胞（PBN）进行两种诊断的鉴别时，灵敏度为100%，特异性为90.0%。这意味着结合pg代谢物和PBN分析可以区分脓膜增生和CEH病例。如果母狗体内的pg代谢物水平为>= 4524 pmol l(-1)，则有99%的可能性诊断为脓膜增生而不是CEH。pg代谢物>= 3054 pmol l(-1), >= 2388 pmol l（-1）或>= 1666 pmol l（-1）的水平分别表明脓脓率为95%，90%或80%。在pg -代谢物水平较高时（高于约3000 pmol l(-1)），单独的pg -代谢物足以区分脓脓症与CEH。本研究结果表明，pg代谢物分析在诊断和预测子宫疾病严重程度方面具有重要价值。(c) 2005爱思唯尔公司版权所有。
Bitches with pyometra are potential emergency cases which may be clinically difficult to differentiate from cases of cystic endometrial hyperplasia (CEH) in combination with mucometra. In the present study plasma prostaglandin F-2 alpha, as measured by its main metabolite 15-keto-13,14-dihydro-PGF(2 alpha) (PG-metabolite) concentrations, blood biochemical and hematological parameters were measured in 59 bitches with pyometra, 10 bitches with CEH and nine controls to determine if PG-metabolite could differentiate between the three uterine conditions. Bitches with pyometra had significantly higher plasma levels of PG-metabolite than bitches with CEH (P = 0.002) and the controls (P = 0.002). PG-metabolite analysis alone had a high sensitivity (98.3%) and a high specificity (80.0%) for the differentiation of pyometra versus CEH in bitches where fluid in the uterus was diagnosed. When a combination of PG-metabolite and percentage band neutrophils (PBN) was used for differentiation of the two diagnoses, a sensitivity of 100% and specificity of 90.0% was obtained. This means that the combination of PG-metabolite and PBN analysis allows for differentiation between cases of pyometra and CEH. If the PG-metabolite level in a bitch is >= 4524 pmol l(-1), there is a 99% probability of the diagnosis pyometra versus CEH. Levels of PG-metabolite >= 3054 pmol l(-1), >= 2388 pmol l(-1) or >= 1666 pmol l(-1) indicates a 95%, 90% or 80% probability of pyometra, respectively. At high PG-metabolite levels (above about 3000 pmol l(-1)), PG-metabolite alone is enough for differentiation of pyometra versus CEH. The results of the present study showed that PG-metabolite analysis is valuable in the diagnosis and prediction of severity of uterine diseases. (c) 2005 Elsevier Inc. All rights reserved.