THE NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF THE ENCEPHALOMYOCARDITIS VIRAL POLYPROTEIN CODING REGION
THE NUCLEOTIDE AND DEDUCED AMINO-ACID-SEQUENCES OF THE ENCEPHALOMYOCARDITIS VIRAL POLYPROTEIN CODING REGION
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DOI：
10.1093/nar/12.6.2969
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
COLLETT, MS
中科院分区：
文献类型：
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作者：
PALMENBERG, AC;KIRBY, EM;COLLETT, MS
确定了脑心肌炎 (EMC) 病毒 RNA 的 7200 个碱基的核苷酸序列，包括完整的多蛋白编码区。该多蛋白在独特的翻译阅读框内编码，长度为 6870 个碱基。蛋白质合成以序列 Met-Ala-Thr 开始，以序列 Leu-Phe-Trp 结束，距 RNA 3' 端 126 个碱基。将病毒衣壳和非衣壳蛋白与多蛋白的推导氨基酸序列进行比对。蛋白水解加工图遵循标准的 4-3-4 小核糖核酸病毒模式，除了衣壳蛋白之前的短前导肽 (8 kd) 之外。蛋白水解切割位点的鉴定表明，EMC 病毒蛋白酶 p22 对具有相邻脯氨酸残基的 gln-gly 或 gln-ser 序列具有切割特异性。宿主编码的蛋白酶的切割特异性包括 tyr-pro 和 gln-gly 序列。
The nucleotide sequence of 7200 bases of encephalomyocarditis (EMC) viral RNA, including the complete polyprotein-coding region, was determined. The polyprotein is encoded within a unique translational reading frame, 6870 bases in length. Protein synthesis begins with the sequence Met-Ala-Thr, and ends with the sequence Leu-Phe-Trp, 126 bases from the 3′ end of the RNA. Viral capsid and noncapsid proteins were aligned with the deduced amino acid sequence of the polyprotein. The proteolytic processing map follows the standard 4-3-4 picornaviral pattern except for a short leader peptide (8 kd), which precedes the capsid proteins. Identification of the proteolytic cleavage sites showed that EMC viral protease, p22, has cleavage specificity for gln-gly or gln-ser sequences with adjacent proline residues. The cleavage specificity of the host-coded protease(s) includes both tyr-pro and gln-gly sequences.