Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis
Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis
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DOI：
10.1007/s10493-009-9245-3
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发表时间：
2009-08-01
影响因子：
2.2
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Imamura, Saiki;Vaz, Itabajara da Silva, Jr.;Ohashi, Kazuhiko
我们报道了长角血蜱金属蛋白酶（HLMP1）的克隆、表达和鉴定。该基因编码一种预测的550个氨基酸的蛋白质，与重溶酶家族的金属蛋白酶相似。该蛋白序列包含一个信号序列，锌结合基序（HEXXHXXGXXH）与金属蛋白酶和富含半胱氨酸的区域共同。逆转录- pcr表达分析表明，mRNA存在于蜱幼虫、若虫和成蜱的唾液腺中。多次感染长角锥虫的家兔能够识别出rHLMP1，提示对HLMP1的免疫反应是通过蜱的摄食自然诱导的。兔接种rHLMP1可产生对蜱虫的保护性免疫，蜱虫若虫和成蜱的死亡率分别为15.6%和14.6%。这项工作为了解蜱虫的防御系统提供了信息，并为开发基于金属蛋白酶的抗蜱疫苗阻断该防御系统的策略提供了新的见解。
We report the cloning, expression and characterization of an Haemaphysalis longicornis metalloprotease (named HLMP1). The gene encodes a predicted 550 aminoacid protein with similarity to metalloproteases of the reprolysin family. The protein sequence contains a signal sequence, the zinc-binding motif (HEXXHXXGXXH) common to metalloproteases and a cysteine-rich region. Reverse transcription-PCR expression analysis indicates the presence of mRNA in the salivary gland of larva, nymph and adult ticks. Rabbit repeatedly infested with H. longicornis recognized rHLMP1, suggesting that the immune-response against HLMP1 is naturally induced through the feeding of ticks. Vaccination of rabbit with rHLMP1 produced protective immunity against ticks, resulting in 15.6 and 14.6% mortality in nymph and adult ticks, respectively. This work provides information to understand the tick's defense system, and offers new insights to develop strategies to block this defense system with an anti-tick vaccine based on a metalloprotease.