RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN ORYZA-SATIVA-L
RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN ORYZA-SATIVA-L
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DOI：
10.1139/g89-563
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发表时间：
1989-12-01
期刊：
影响因子：
3.1
通讯作者：
TANKSLEY, SD
中科院分区：
文献类型：
--
作者：
WANG, ZY;TANKSLEY, SD
利用10个水稻限制性片段长度多态性标记对代表水稻品种宽度的70个水稻品种进行了分析。所有探针都检测到多态性，并且通过所有探针-酶组合可以唯一地区分70个品种中的58个。在26%的品种中发现了群体内变异，通常以纯合变异等位基因的形式出现。根据遗传距离计算，水稻品种间和品种内的遗传变异比例约为12:1。基于限制性片段长度多态性的树状图也被构建来描述水稻品种之间的遗传距离。
Seventy varieties of rice, representing the breadth of the species Oryza sativa, were assayed with 10 rice restriction fragment length polymorphism makers. Polymorphism was detected for all probes and 58 of 70 varieties tested could be uniquely distinguished from one another by combining all probe-enzyme combinations. Within-population variation, usually in the form of homozygous variants alleles, was found for 26% of the varieties. Based on genetic distance calculations, the ratio of the genetic variation between versus within rice varieties was estimated to be approximately 12:1. A restriction fragment length polymorphism based dendrogram has also been constructed depicting genetic distances among rice varieties.