Detection of pathogen Escherichia coli O157:H7 with a wireless magnetoelastic-sensing device amplified by using chitosan-modified magnetic Fe3O4 nanoparticles
Detection of pathogen Escherichia coli O157:H7 with a wireless magnetoelastic-sensing device amplified by using chitosan-modified magnetic Fe3O4 nanoparticles
复制标题
使用壳聚糖修饰的磁性 Fe3O4 纳米颗粒放大的无线磁弹性传感装置检测病原体大肠杆菌 O157:H7
DOI：
10.1016/j.snb.2010.03.011
复制
发表时间：
2010-05-18
影响因子：
8.4
通讯作者：
Grimes, Craig A.
中科院分区：
文献类型：
--
作者：
Lin, Hailan;Lu, Qingzhu;Grimes, Craig A.
本文报道了一种用于大肠杆菌O 157：H7（Escherichia con O 157：H7）病原体检测的磁弹性传感装置。coli）为靶点，以壳聚糖修饰的磁性Fe 3 O 4纳米粒子（CMNPs）为信号放大标签。在合适的pH值下，CMNPs，用壳聚糖层功能化的Fe 3 O 4纳米颗粒，结合到带负电荷的E。大肠杆菌通过静电吸引。急诊coli附着的CMNP磁性结合到磁致弹性传感器的表面，导致传感器表面上的质量负载增强，这反过来降低了其共振频率，从而允许定量E.大肠杆菌浓度。该传感器对大肠杆菌的对数浓度有线性响应。大肠杆菌的最低检测限（LOD）为10 cells mL（-1）~ 3.7 x 10（8）cells mL（-1）。牛血清白蛋白、卵清蛋白等小分子物质对检测无干扰。(C)2010 Elsevier B. V.保留所有权利。
We report on a wireless magnetoelastic-sensing device for the assay of pathogen with Escherichia con O157:H7 (E. coli) as a target using chitosan-modified magnetic Fe3O4 nanoparticles (CMNPs) as signal-amplifying tags. At suitable pH the CMNPs, Fe3O4 nanoparticles functionalized with a layer of chitosan, bind to negatively charged E. coli through electrostatic attraction. The E. coli attached CMNPs are magnetically bound to the surface of the magnetoelastic sensor, resulting in enhanced mass loading on the sensor surface that in turn decreases its resonance frequency allowing quantification of E. coli concentrations. The sensor shows a linear response to the logarithmic concentration of E. coli in the range of 10 cells mL(-1) to 3.7 x 10(8) cells mL(-1) with a detection limit (LOD) of 10 cells mL(-1). Small molecules such as albumin bovine and ovalbumin show no interference on the detection. (C) 2010 Elsevier B.V. All rights reserved.