Differential diagnosis of Taenia saginata and Taenia saginata asiatica taeniasis through PCR
Differential diagnosis of Taenia saginata and Taenia saginata asiatica taeniasis through PCR
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DOI：
10.1016/j.diagmicrobio.2004.03.013
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发表时间：
2004-07-01
影响因子：
2.9
通讯作者：
Gárate, T
中科院分区：
文献类型：
--
作者：
González, LM;Montero, E;Gárate, T
根据牛带绦虫HDP 2 DNA序列（AccN#AJ133740）设计了一种新的多重PCR和PCR连锁限制性片段长度多态性方法，可以同时特异性地鉴定牛带绦虫。牛带绦虫Taenia saginata asiatica。从20个不同的西班牙绦虫病患者排出节片，以前诊断为T。采用形态学鉴定和多重HDP 2-PCR技术对Saginata进行了鉴定，并采用新开发的PCR技术对T.证实感染Saginata。所有的20个T。Saginata样品在T.刀豆特异性PCR三个真实的T。saginata asiatica样品在T. Saginata asiatica这些新的协议有直接的潜力，特异性，敏感性和快速识别T。saginata asiatica，可能有助于分类研究。(C)2004年爱思唯尔公司All rights reserved.
New multiplex-PCR and PCR-linked restriction fragment length polymorphism protocols, derived from Taenia saginata HDP2 DNA sequence (AccN#AJ133740), have been designed that allow the simultaneous and specific identification of T. saginata and Taenia saginata asiatica. Proglottids expelled from 20 different Spanish taeniasis patients, previously diagnosed as T. saginata by both morphological identification and multiplex HDP2-PCR, were also examined by the newly developed PCR protocols, and the original diagnosis of T. saginata infection was confirmed. All of the 20 T. saginata samples were negative in the T. saginata asiatica-specific PCR. Three authentic T. saginata asiatica samples were unambiguously identified as such in the T. saginata asiatica PCR. These new protocols have immediate potential for the specific, sensitive, and rapid identification of T. saginata asiatica and may assist in taxonomic studies. (C) 2004 Elsevier Inc. All rights reserved.