Array-based electrical detection of DNA with nanoparticle probes
Array-based electrical detection of DNA with nanoparticle probes
复制标题
DOI：
10.1126/science.1067003
复制
发表时间：
2002-02-22
期刊：
影响因子：
56.9
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Park, SJ;Taton, TA;Mirkin, CA
报道了一种DNA阵列检测方法，其中用金纳米颗粒官能化的寡核苷酸的结合导致与靶-探针结合事件相关的电导率变化。结合事件将金纳米颗粒定位在电极间隙中;由这些纳米颗粒促进的银沉积桥接差距并导致容易测量的电导率变化。利用与这些纳米颗粒探针相关的不寻常的盐浓度依赖性杂交行为来实现选择性而无需热严格洗涤。使用这种方法，我们已经检测到浓度低至500毫微微摩尔的目标DNA，点突变选择性因子类似于100，000：1。
A DNA array detection method is reported in which the binding of oligonucleotides functionalized with gold nanoparticles leads to conductivity changes associated with target-probe binding events. The binding events localize gold nanoparticles in an electrode gap; silver deposition facilitated by these nanoparticles bridges the gap and leads to readily measurable conductivity changes. An unusual salt concentration-dependent hybridization behavior associated with these nanoparticle probes was exploited to achieve selectivity without a thermal-stringency wash. Using this method, we have detected target DNA at concentrations as low as 500 femtomolar with a point mutation selectivity factor of similar to 100,000:1.