Influence of Brownian Rotations and Energy Transfer upon the Measurements of Fluorescence Lifetime
Influence of Brownian Rotations and Energy Transfer upon the Measurements of Fluorescence Lifetime
复制标题
布朗旋转和能量转移对荧光寿命测量的影响
DOI：
10.1063/1.1673201
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发表时间：
1970
影响因子：
4.4
通讯作者：
G. Weber
中科院分区：
文献类型：
--
作者：
R. Spencer;G. Weber
通过布朗旋转或分子间能量转移的溶液的荧光的去偏振可以简单地通过一阶线性微分方程系统来描述，该系统仅包含荧光发射速率和激发从发射的一个正交分量到另一个正交分量的传输速率作为参数。稳态解的形式为Perrin方程，描述了布朗旋转的去偏振，而单位光脉冲后的时间依赖性去偏振最初由Jablonski描述。正弦激励的解决方案是新颖的，因为：1。它表明发射的偏振分量之间的寿命差异是调制频率ω与发射率λ之比的敏感函数。对于ω/λ > 1，偏振寿命之间的差异可能比在单位光脉冲之后观察到的差异大许多倍。2.它允许确定两个速率的transp…
The depolarization of the fluorescence of solutions by either Brownian rotations or intermolecular energy transfer may be simply described by a system of first‐order linear differential equations containing as only parameters the rate of fluorescence emission and the rate of transport of the excitation from one orthogonal component of the emission to another. The steady‐state solution has the form of Perrin's equation describing the depolarization by Brownian rotations, and the time‐dependent depolarization following a unit light impulse is that originally described by Jablonski. The solution for sinusoidal excitation is novel in that: 1. It shows the difference in lifetime between the polarized components of the emission to be a sensitive function of the ratio of the modulation frequency ω to the emission rate λ. For ω/λ > 1 the difference between the polarized lifetimes may become many times greater than that observed after a unit light impulse. 2. It permits the determination of both the rate of transp...