The loss of potassium from the erythrocyte in hypotonic saline
The loss of potassium from the erythrocyte in hypotonic saline
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DOI：
10.1002/jcp.1030100209
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发表时间：
1937-10-01
影响因子：
--
通讯作者：
Davson, H
中科院分区：
文献类型：
--
作者：
Davson, H
本文研究了几种哺乳动物红细胞在40 ℃低渗溶液中钾的损失。在此条件下，所有物种的钾素损失率为：兔、马、牛>豚鼠>猪、人。K的损失率对介质张力的曲线是S形的，表明存在一个临界体积，给定的细胞在变得可渗透K之前必须达到该临界体积。当膨胀的细胞失去足够的K，使其收缩到这个临界体积时，K的损失从这个细胞停止，因此最终浓度。当钾的损失停止时，细胞中的钾含量仍远高于悬浮培养基中的钾含量。随后稀释的悬浮液介质导致K的损失再次开始。这种影响并不只是温度所特有的。40、“以不变应万变，以不变应万变”。在兔子的情况下，可检测的损失发生在20[度];温度的近似估计。系数在该物种的情况下，给出约2的值。通过直接实验排除了盐溶液中重金属杂质造成钾损失的可能性。结论是它们是由于红细胞膜的机械拉伸。
The losses of K from the erythrocytes of several species of mammals in hypotonic solutions at 40[degree] were studied. All the species studied lose K under these conditions and their relative rates are in the order: rabbit, horse, ox > guinea pig> pig, human. The plot of rate of loss of K against the tonicity of the medium is S-shaped indicating that there is a critical volume which a given cell must attain before it becomes permeable to K. When the swollen cell loses sufficient K to allow of its shrinking to this critical volume the loss of K ceases from this cell, so that the final concn. of K in the cells when the loss of K has ceased is still much higher than that in the suspension medium. Subsequent dilution of the suspension medium causes the loss of K to start again. The effect is not peculiar to the temp. of 40[degree] but varies continuously with the temp., and in the case of the rabbit detectable losses occur at 20[degree]; an approximate estimate of the temp. coeff. in the case of this species gives a value of about 2. The possibility that the losses of K are due to heavy metal impurities in the saline solutions is excluded by direct expts.; it is concluded that they are due to a mechanical stretching of the erythrocyte membrane.