The role of Ca2+ in dimethyl sulfoxide-induced differentiation of Friend erythroleukemia cells.
The role of Ca2+ in dimethyl sulfoxide-induced differentiation of Friend erythroleukemia cells.
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Ca2 在二甲基亚砜诱导 Friend 红白血病细胞分化中的作用。
DOI：
10.1016/s0021-9258(18)89154-2
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
I. Macara
中科院分区：
文献类型：
--
作者：
D. Faletto;I. Macara
培养的Friend细胞可以被二甲基亚砜（Me 2SO）和几种其它试剂诱导沿着红系途径成熟沿着。已有证据表明，Ca 2+流入的增加是这些细胞成熟的早期和必要的前奏（Levenson，R.，豪斯曼，D.，和Cantley，L.等人（1980）Proc. Acad. Sci. U.S.A.77，5948-5952）。支持所有可用数据的最简单假设是Me 2SO和其他诱导剂升高胞质Ca 2+浓度。我们现在已经使用荧光指示剂quin-2测量了胞质Ca 2+，并且发现，与预期相反，用Me 2SO处理细胞后，Ca 2+略有下降。胞浆Ca 2+的增加，通过提高培养基中的Ca 2+，但没有显着改变通过添加哇巴因或莫能菌素或通过在Na+-无培养基中孵育。用3 H2O和125 I-白蛋白分别测定细胞水和细胞外空间的三重标记技术测定细胞总钙，结果显示用Me 2SO处理40 h后无显著变化。当在4 ℃下测量时，在Me 2SO处理的细胞中观察到45 Ca 2+流入的初始速率降低。这些数据不支持的假设，即细胞内钙离子的增加是必要的诱导的朋友细胞分化或Na+/Ca 2+交换是一个显着的调节剂的胞质Ca 2+在朋友细胞。
Cultured Friend cells can be induced by dimethyl sulfoxide (Me2SO) and several other agents to mature along the erythroid pathway. Evidence has been presented that an increase in Ca2+ influx is an early and necessary prelude to the commitment to maturation by these cells (Levenson, R., Housman, D., and Cantley, L. (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 5948-5952). The simplest hypothesis supporting all the available data is that Me2SO and other inducers elevate the cytosolic Ca2+ concentration. We have now measured cytosolic Ca2+ using the fluorescent indicator quin-2, and find, contrary to expectation, a small decrease upon treatment of cells with Me2SO. Cytosolic Ca2+ was increased by raising the Ca2+ in the medium, but was not dramatically altered by addition of ouabain or monensin or by incubation in Na+-free medium. Measurement of total cell Ca2+ by a triple-labeling technique using 3H2O and 125I-albumin to determine cell water and extracellular space, respectively, revealed no significant change upon treatment with Me2SO for up to 40 h. A decrease in the initial rate of 45Ca2+ influx was observed in Me2SO-treated cells, when measured at 4 degrees C. These data do not support the hypothesis that an increase in cell Ca2+ is necessary for the induction of Friend cell differentiation or that Na+/Ca2+ exchange is a significant regulator of cytosolic Ca2+ in Friend cells.