Genome-wide analysis of signaling networks regulating fatty acid-induced gene expression and organelle biogenesis
Genome-wide analysis of signaling networks regulating fatty acid-induced gene expression and organelle biogenesis
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DOI：
10.1083/jcb.200710009
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发表时间：
2008-04-21
影响因子：
7.8
通讯作者：
Aitchison, John D.
中科院分区：
文献类型：
--
作者：
Saleem, Ramsey A.;Knoblach, Barbara;Aitchison, John D.
可逆磷酸化是最常见的翻译后修饰。用于调节细胞过程的阳离子。这项研究所需的过氧化物酶体生物合成的磷酸酶和激酶是第一个全基因组的磷酸化事件控制细胞器生物合成的分析。我们评估信号分子缺失菌株的酵母酿酒酵母过氧化物酶体硫解酶，过氧化物酶体的形成，过氧化物酶体功能的绿色荧光蛋白嵌合体的存在。我们发现，不同的信号网络，涉及葡萄糖介导的过氧化物酶体形成促进阶段的生物合成途径。此外，不同类别的信号蛋白负责过氧化物酶体数量和大小的调节。这些信号网络指定过氧化物酶体生物发生的早期和晚期事件的要求。在参与的众多信号蛋白中，Pho85 p是例外的，在基因表达和过氧化物酶体形成中具有功能参与。我们的研究代表了第一个全球性的信号网络调节细胞器的生物发生的研究。
Reversible phosphorylation is the most common posttranslational modi. cation used in the regulation of cellular processes. This study of phosphatases and kinases required for peroxisome biogenesis is the first genome-wide analysis of phosphorylation events controlling organelle biogenesis. We evaluate signaling molecule deletion strains of the yeast Saccharomyces cerevisiae for presence of a green fluorescent protein chimera of peroxisomal thiolase, formation of peroxisomes, and peroxisome functionality. We find that distinct signaling networks involving glucose-mediated and peroxisome formation promote stages of the biogenesis pathway. Additionally, separate classes of signaling proteins are responsible for the regulation of peroxisome number and size. These signaling networks specify the requirements of early and late events of peroxisome biogenesis. Among the numerous signaling proteins involved, Pho85p is exceptional, with functional involvements in both gene expression and peroxisome formation. Our study represents the first global study of signaling networks regulating the biogenesis of an organelle.