cAMP induces a rapid and reversible modification of the chemotactic receptor in Dictyostelium discoideum.
cAMP induces a rapid and reversible modification of the chemotactic receptor in Dictyostelium discoideum.
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DOI：
10.1083/jcb.100.3.715
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发表时间：
1985-03
影响因子：
7.8
通讯作者：
Simons, S
中科院分区：
文献类型：
--
作者：
Klein, C;Lubs-Haukeness, J;Simons, S
在cAMP刺激后1分钟内，发现有聚集能力的盘基网柄藻的刺激引起细胞表面cAMP受体的快速（1分钟内）修饰。修饰的受体在SDS PAGE上作为47，000-mol-wt蛋白迁移，而在未刺激的细胞上标记的蛋白为45，000-mol-wt。这种修饰的受体被检测到的时间长短取决于cAMP刺激的强度：用10（-7）M cAMP处理后3-4分钟，细胞不再具有47，000-mol-wt形式的cAMP受体。相反，存在45，000-mol-wt形式。然而，用10（-5）M cAMP刺激细胞，导致修饰受体的持续表达（超过15分钟）。发现cAMP受体中cAMP诱导的移位的时间过程、浓度依赖性和刺激特异性与cAMP刺激的47，000-mol-wt蛋白质的磷酸化平行。此外，这两种现象被证明发生在内源性cAMP合成的情况下。cAMP受体磷酸化的可能性，在响应cAMP刺激，和细胞脱敏的作用，这一事件进行了讨论。
Stimulation, within 1 min after cAMP stimulation, of aggregation- competent Dictyostelium discoideum amebae was found to cause a rapid (within 1 min) modification of the cell's surface cAMP receptor. The modified receptor migrated on SDS PAGE as a 47,000-mol-wt protein, as opposed to a 45,000-mol-wt protein labeled on unstimulated cells. The length of time this modified receptor could be detected depended upon the strength of the cAMP stimulus: 3-4 min after treatment with 10(-7) M cAMP, cells no longer possessed the 47,000-mol-wt form of the cAMP receptor. Instead, the 45,000-mol-wt form was present. Stimulation of cells with 10(-5) M cAMP, however, resulted in the persistent (over 15 min) expression of the modified receptor. The time course, concentration dependence, and specificity of stimulus for this cAMP- induced shift in the cAMP receptor were found to parallel the cAMP- stimulated phosphorylation of a 47,000-mol-wt protein. In addition, both phenomena were shown to occur in the absence of endogenous cAMP synthesis. The possibility that the cAMP receptor is phosphorylated in response to cAMP stimulation, and the role of this event in cell desensitization, are discussed.