Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging
Preliminary Study on Diffuse Axonal Injury by Fourier Transform Infrared Spectroscopy Histopathology Imaging
复制标题
DOI：
10.1111/1556-4029.12290
复制
发表时间：
2014-01
影响因子：
1.6
通讯作者：
Tiantong Yang;G. He;Xiang Zhang;Lin Chang;Hai-dong Zhang;M. Ripple;David R. Fowler;Ling Li
中科院分区：
文献类型：
--
作者：
Tiantong Yang;G. He;Xiang Zhang;Lin Chang;Hai-dong Zhang;M. Ripple;David R. Fowler;Ling Li
本研究的目的是评价傅里叶变换红外光谱（FTIR）在小鼠弥漫性轴索损伤（DAI）检测中的应用。采用H&E、银和β -淀粉样前体蛋白（β - app）免疫组化染色制备DAI小鼠模型脑组织，并用FTIR进行研究。红外光谱图像显示，实验小鼠大脑皮层下白质对酰胺II有较高的吸收，而对照组小鼠大脑中酰胺II无明显表达。高吸收酰胺II的区域分布与银和β - APP研究证实的DAI区域相同。结果表明，高吸收与轴索损伤有关。使用FTIR成像可以检测组织中与DAI病理相关的生化变化，从而为当前常规病理诊断技术提供了重要的辅助方法。
The objective of this study was to evaluate the application of Fourier transform infrared (FTIR) spectroscopy for detecting diffuse axonal injury (DAI) in a mouse model. Brain tissues from DAI mouse model were prepared with H&E, silver, and β‐amyloid precursor protein (β–APP) immunohistochemistry stains and were also studied with FTIR. The infrared spectrum images showed high absorption of amide II in the subcortical white matter of the experimental mouse brain, while there was no obvious expression of amide II in the control mouse brain. The areas with high absorption of amide II were in the same distribution as the DAI region confirmed by the silver and β‐APP studies. The result suggests that high absorption of amide II correlates with axonal injury. The use of FTIR imaging allows the biochemical changes associated with DAI pathologies to be detected in the tissues, thus providing an important adjunct method to the current conventional pathological diagnostic techniques.