Effect of repeated antigen exposure on antigen-and mediator-induced bronchospasm in sheep.
Effect of repeated antigen exposure on antigen-and mediator-induced bronchospasm in sheep.
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反复接触抗原对绵羊抗原和介质诱导的支气管痉挛的影响。
DOI：
10.1152/jappl.1985.59.6.1866
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Spannhake,EW
中科院分区：
文献类型：
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作者：
Kleeberger,SR;Wagner,EM;Adams3rd,GK;DannenbergJr,AM;Spannhake,EW
我们研究了反复暴露抗原对气道对过敏反应介质的反应性和对抗原的即时反应的影响。7只抗原敏感羊每两周暴露于猪蛔虫抗原气溶胶5次；对照组7只羊采用相同的盐水载药暴露方案。绵羊被分为实验组（蛔虫）和对照组，因此动物对介质的支气管反应性分布大致相同。通过测定雾化组胺（10 - 1000微克）、前列腺素F2 α （PGF2 α， 10-300微克）和稳定的血栓素A2类似物（U-46619, 1-100微克）对肺阻力（RL）和动态肺顺应性（Cdyn）的影响来评估气道反应性。治疗前，实验组和对照组RL和Cdyn的变化相似，相似物大于组胺，大于PGF2 α。在每种激动剂的最高剂量下，RL的平均增幅分别为50%、123和29%，Cdyn的平均降幅分别为21%、45%和12%。在暴露于抗原气溶胶的前15分钟内，平均RL增加了125%，Cdyn下降了基线值的38%；暴露后的恶性通货膨胀将这一变化分别减少到56%和31%。RL和Cdyn在最终抗原暴露期间和暴露后恶性膨胀后的变化与初始暴露相比显著降低（P < 0.05）。暴露于抗原或生理盐水前后的基线RL和Cdyn无显著差异。在研究的剂量范围内，这些阿托品化动物对组胺、PGF2 α或类似物的气道反应性没有显著改变。（摘要删节250字）
We studied the effects of repeated exposures of antigen on airway reactivity to mediators of anaphylaxis and immediate response to the antigen. Seven antigen-sensitive sheep were exposed to aerosols of Ascaris suum antigen 5 times biweekly; a control group of seven sheep underwent the same exposure regimen with saline vehicle. Sheep were assigned to experimental (Ascaris) or control groups so the distribution of animals with regard to bronchial reactivity to mediators was about the same. Airway reactivity was assessed by determining the effects of aerosolized histamine (10–1,000 micrograms), prostaglandin F2 alpha (PGF2 alpha, 10–300 micrograms), and a stable analogue of thromboxane A2 (U-46619, 1–100 micrograms) on lung resistance (RL) and dynamic lung compliance (Cdyn). Before treatment, experimental and control groups showed similar changes in RL and Cdyn, with analogue greater than histamine greater than PGF2 alpha. At the highest dose of each agonist, mean increases in RL were 50, 123, and 29%, respectively, and mean decreases in Cdyn were 21, 45, and 12%. During the first 15-min exposures to antigen aerosol, mean RL had increased by 125% and Cdyn decreased by 38% of base-line values; hyperinflation following the exposures reduced the changes to 56 and 31%, respectively. Changes in RL and Cdyn during the final antigen exposures and following postexposure hyperinflation were reduced significantly (P less than 0.05) compared with the initial exposures. Baseline RL and Cdyn before and after the exposures to antigen or saline were not significantly different. Airway reactivity to histamine, PGF2 alpha, or analogue was not significantly altered in these atropinized animals over the range of doses studied.(ABSTRACT TRUNCATED AT 250 WORDS)