Species diversity and relative abundance of vaginal lactic acid bacteria from women in Uganda and Korea
Species diversity and relative abundance of vaginal lactic acid bacteria from women in Uganda and Korea
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DOI：
10.1111/j.1365-2672.2006.03147.x
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发表时间：
2007-04-01
影响因子：
4
通讯作者：
Wawer, M.
中科院分区：
文献类型：
--
作者：
Jin, L.;Tao, L.;Wawer, M.
目的：乳酸菌在维持女性阴道健康中起着重要作用。本研究的目的是比较两个地理位置遥远的国家，乌干达和韩国的妇女乳酸菌和其他乳酸菌的物种丰富度和相对丰度。方法和结果：从乌干达和韩国的两个妇女人群中获得阴道样本。采用Rogosa乳杆菌SL琼脂对乳酸菌进行初步分离。经表型分析后，采用聚合酶链反应扩增16S rRNA基因，并通过BLAST程序和系统发育树构建进行分析。共分离出阴道乳酸菌338株（韩国128株，乌干达210株），包括乳杆菌属、白乳酸菌属、Pediococcus、Streptococcus和Weissella 5属。虽然在两种人群中都有criscrisus乳杆菌，但发酵乳杆菌仅在韩国女性中常见，而产气乳杆菌、罗伊氏乳杆菌和阴道乳杆菌仅在乌干达女性中常见。在其他乳酸菌中，韦塞拉乳酸菌在乌干达更常见，Pediococcus在韩国女性中更常见。所有的魏氏菌菌株都产生过氧化氢，所有的Pediococcus菌株都抑制念珠菌。结论：虽然有许多乳酸菌定植于女性，但在地理上不同的群落中，其种类分布可能不同。研究的意义和影响：了解阴道乳酸菌的物种丰富度和相对丰度，包括乳杆菌、Pediococcus和Weissella，可能有助于设计更好的益生菌产品作为细菌替代疗法。
Aims: Lactobacilli play an important role in maintaining vaginal health of women. The aim of this study was to compare the species richness and relative abundance of Lactobacillus and other lactic acid bacteria in women of two geographically distant countries, Uganda and Korea.Methods and Results: Vaginal samples were obtained from two women populations in Uganda and Korea. The Lactobacillus Rogosa SL agar was used for initial isolation of lactic acid bacteria. After phenotypic analyses, the 16S rRNA gene was amplified by polymerase-chain reaction and analysed by the BLAST program and phylogenetic tree construction. A total of 338 (128 Korean and 210 Ugandan) vaginal lactic acid bacterial strains were isolated, including five genera: Lactobacillus, Leuconostoc, Pediococcus, Streptococcus and Weissella. While Lactobacillus crispatus was common in both populations, Lactobacillus fermentum was common only in Korean women, and Lactobacillus gasseri, Lactobacillus reuteri and Lactobacillus vaginalis only in Ugandan women. Among other lactic acid bacteria, Weissella was more common in Ugandan, and Pediococcus in Korean women. All Weissella strains produced hydrogen peroxide, and all Pediococcus strains inhibited Candida species.Conclusion: Although many lactic acid bacteria colonize women, their species distributions may be different in women of geographically separated communities.Significance and Impact of the Study: The knowledge of species richness and relative abundance of vaginal lactic acid bacteria, including Lactobacillus, Pediococcus and Weissella, may lead to the design of better probiotic products as bacterial replacement therapy.