Extracellular N-domain alone can mediate specific heterophilic adhesion between members of the carcinoembryonic antigen family, CEACAM6 and CEACAM8
Extracellular N-domain alone can mediate specific heterophilic adhesion between members of the carcinoembryonic antigen family, CEACAM6 and CEACAM8
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DOI：
10.1006/bbrc.2000.3858
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发表时间：
2000-11-30
影响因子：
3.1
通讯作者：
Nakazato, H
中科院分区：
文献类型：
--
作者：
Oikawa, S;Sugiyama, M;Nakazato, H
利用表达嵌合抗原的中国仓鼠卵巢（CHO）转染物，研究了癌胚抗原（CEA）家族的两个成员CEACAM6和CEACAM8之间具有特异性异源性粘附的结构域(s)，这两个结构域都具有三个细胞外结构域。利用将CEACAM3的唯一胞外结构域（N-domain）替换为CEACAM6的细胞外结构域（N-domain）的嵌合抗原，证明了CEACAM6的N-domain能够介导CEACAM8的特异性粘附。此外，嵌合抗原与n结构域被CEACAM8取代的嵌合CEA结合显著，而与未被取代的CEA不结合。这些结果表明，仅n结构域是足够的，CEACAM6或CEACAM8的其他结构域不需要这种特异性结合。因此，我们提出了一种不同于CEA-CEA亲同结合的n域间的异亲相互作用模型。(C) 2000年学术出版社。
The domain(s) responsible for the specific heterophilic adhesion between two members of the carcinoembryonic antigen (CEA) family, CEACAM6 and CEACAM8, both of which with three extracellular domains, mere investigated using Chinese hamster ovary (CHO) transfectants expressing chimeric antigens. Using a chimeric antigen in which the N-domain, a sole extracellular domain, of CEACAM3 was substituted with that of CEACAM6, it was shown that the N-domain of CEACAM6 alone was able to mediate specific adhesion to CEACAM8. Furthermore, the chimeric antigen was shown to bind significantly to chimeric CEA whose N-domain was substituted with that of CEACAM8, but not to unsubstituted CEA. These results demonstrate that the N-domain alone is sufficient and other domains of CEACAM6 or CEACAM8 are not required for this specific binding. We therefore propose a model of heterophilic interaction between the N-domains, which is distinct from that of CEA-CEA homophilic binding. (C) 2000 Academic Press.