Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells.
Bioabsorbable zinc ion induced biphasic cellular responses in vascular smooth muscle cells.
复制标题
DOI：
10.1038/srep26661
复制
发表时间：
2016-06-01
影响因子：
4.6
通讯作者：
Zhu D
中科院分区：
文献类型：
--
作者：
Ma J;Zhao N;Zhu D
生物可吸收金属锌（Zn）是一种具有广阔应用前景的新一代植入式支架材料。在心血管支架应用中，锌离子（Zn2+）在植入后会逐渐从含锌支架中释放到周围血管组织中。然而，血管细胞与Zn2+之间的相互作用在很大程度上仍然未知。我们探索了细胞外Zn2+对人平滑肌细胞（SMCs） 24小时内的短期影响，并观察到Zn2+有趣的双相效应。较低浓度（<80 μM）的Zn2+对细胞活力无不良影响，但促进细胞粘附、细胞扩散、细胞增殖、细胞迁移，并增强F-actin和vinculin的表达。与未经任何处理的对照组相比，用如此低浓度的Zn2+处理的细胞显示出细长的形状。相比之下，较高Zn2+浓度（80 ~ 120 μM）处理的细胞有相反的细胞反应和行为。基因表达谱显示，受影响最大的功能基因与血管生成、炎症、细胞粘附、血管张力和血小板聚集有关。结果表明，锌对SMCs具有明显的浓度依赖性双相作用，低浓度对细胞功能有利。
Bioabsorbable metal zinc (Zn) is a promising new generation of implantable scaffold for cardiovascular and orthopedic applications. In cardiovascular stent applications, zinc ion (Zn2+) will be gradually released into the surrounding vascular tissues from such Zn-containing scaffolds after implantation. However, the interactions between vascular cells and Zn2+ are still largely unknown. We explored the short-term effects of extracellular Zn2+ on human smooth muscle cells (SMCs) up to 24 h, and an interesting biphasic effect of Zn2+ was observed. Lower concentrations (<80 μM) of Zn2+ had no adverse effects on cell viability but promoted cell adhesion, cell spreading, cell proliferation, cell migration, and enhanced the expression of F-actin and vinculin. Cells treated with such lower concentrations of Zn2+ displayed an elongated shape compared to controls without any treatment. In contrast, cells treated with higher Zn2+ concentrations (80–120 μM) had opposite cellular responses and behaviors. Gene expression profiles revealed that the most affected functional genes were related to angiogenesis, inflammation, cell adhesion, vessel tone, and platelet aggregation. Results indicated that Zn has interesting concentration-dependent biphasic effects on SMCs with low concentrations being beneficial to cellular functions.