Characterization of collagen gel solutions and collagen matrices for cell culture
Characterization of collagen gel solutions and collagen matrices for cell culture
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DOI：
10.1016/s0142-9612(00)00315-x
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发表时间：
2001-07-01
期刊：
影响因子：
14
通讯作者：
Ho, HO
中科院分区：
文献类型：
--
作者：
Sheu, MT;Huang, JC;Ho, HO
研究了戊二醛作为交联剂对细胞培养用胶原基质强度的影响。用不同浓度(0~0.2%)的戊二醛处理1%的胶原液24 h，用动态力学分析(DMA)测定胶原凝胶溶液的粘弹性，结果表明所有胶原凝胶溶液遵循相同的模型模式。Voigt-Kelvin的蠕变柔量模型很好地描述了这些胶原凝胶溶液所表达的粘弹性的变化。将这些交联胶原蛋白凝胶溶液冷冻干燥，形成厚度约0.2-0.3 mm的基质。DMA测定的断裂模数表明，交联度越高，断裂模数越高。从裸鼠皮肤分离的成纤维细胞与这些胶原基质的相容性被发现在细胞密度为3×10(5)细胞/厘米(2)时是可接受的，没有收缩，即使在使用浓度高达0.2%的戊二醛时也是如此。(C)2001爱思唯尔科学有限公司。保留所有权利。
The influence of glutaraldehyde as a crosslinking agent to increase the strength of collagen matrices for cell culture was examined in this study. Collagen solutions of 1% were treated with different concentrations (0-0.2%) of glutaraldehyde for 24 h. The viscoelasticity of the resulting collagen gel solution was measured using dynamic mechanical analysis (DMA), which demonstrated that all collagen gel solutions examined followed the same model pattern. The creep compliance model of Voigt-Kelvin satisfactorily described the change of viscoelasticity expressed by these collagen gel solutions. These crosslinked collagen gel solutions were freeze-dried to form a matrix with a thickness of about 0.2-0.3 mm. The break modulus of these collagen matrices measured by DMA revealed that the higher the degree of crosslinking, the higher the break modulus. The compatibility of fibroblasts isolated from nude mouse skin with these collagen matrices was found to be acceptable at a cell density of 3 x 10(5) cells/cm(2) with no contraction, even when using a concentration of glutaraldehyde of up to 0.2%. (C) 2001 Elsevier Science Ltd. All rights reserved.