Gene Expression Profiling Shows That NbFDN1 Is Involved in Modulating the Hypersensitive Response-Like Cell Death Induced by the Oat dwarf virus RepA Protein
Gene Expression Profiling Shows That NbFDN1 Is Involved in Modulating the Hypersensitive Response-Like Cell Death Induced by the Oat dwarf virus RepA Protein
复制标题
基因表达谱显示 NbFDN1 参与调节燕麦矮缩病毒 repa 蛋白诱导的过敏反应样细胞死亡
DOI：
10.1094/mpmi-12-17-0291-r
复制
发表时间：
2018-10-01
影响因子：
3.5
通讯作者：
Zhou, Xueping
中科院分区：
文献类型：
--
作者：
Hou, Huwei;Hu, Ya;Zhou, Xueping
在这项研究中，我们使用高通量的深度核苷酸测序的特点的全局转录响应本塞姆氏烟草植物的RepA蛋白从燕麦矮缩病毒（ODV）的瞬时表达。我们确定了7，878个显著差异表达的基因（DEG），映射到125个途径，这表明全面的网络参与调节RepA诱导的细胞死亡。在与光合作用相关的202个DEG中，发现195个的表达被下调，表明响应于RepA表达的光合作用的显著抑制，这与叶绿体破坏和生理变化相关。我们将我们的分析集中在NbFDN1上，NbFDN1是铁氧还蛋白蛋白家族的一员，参与叶绿体电子传递链进行产氧光合作用，被鉴定为直接与NbTsip 1相互作用。我们使用病毒诱导的基因沉默分别敲低了NbFDN1和NbTsip1的表达，发现NbFDN1沉默加速了RepA诱导的细胞死亡的发展，而不像NbTsip1沉默，它对RepA诱导的反应表现出相反的效果。进一步的研究表明，在NbFDN1沉默的植物中，H2O2积累增加，NbFDN1和NbTsip1的转录本之间呈负相关。因此，我们推测NbFDN1通过调节NbTsip1转录以及H2O2产生对RepA诱导的过敏反应样反应具有影响。
In this study, we used high-throughput deep nucleotide sequencing to characterize the global transcriptional response of Nicotiana benthamiana plants to transient expression of the RepA protein from Oat dwarf virus (ODV). We identified 7,878 significantly differentially expressed genes (DEG) that mapped to 125 pathways, suggesting that comprehensive networks are involved in regulation of RepA-induced cell death. Of the 202 DEG associated with photosynthesis, expression of 195 was found to be downregulated, indicating a significant inhibition of photosynthesis in response to RepA expression, which is associated with chloroplast disruption and physiological changes. We focused our analysis on NbFDN1, a member of the ferredoxin protein family that participates in the chloroplast electron transport chain performing oxygenic photosynthesis, which was identified to directly interact with NbTsip1. We separately knocked down the expression of NbFDN1 and NbTsip1 using virus-induced gene silencing, and found that NbFDN1 silencing speeded up the development of RepA-induced cell death, unlike NbTsip1 silencing, which showed an opposite effect on RepA-induced response. Further study showed increased H2O2 accumulation and a negative correlation between the transcripts of NbFDN1 and NbTsip1 in NbFDN1-silenced plants. Hence, we speculate that NbFDN1 has an effect on RepA-induced hypersensitive response-like response by modulating NbTsip1 transcription as well as H2O2 production.