Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif
Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif
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DOI：
10.1038/s41467-020-15731-w
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发表时间：
2020-04-27
影响因子：
16.6
通讯作者：
Giraldo, Juan Pablo
中科院分区：
文献类型：
--
作者：
Santana, Israel;Wu, Honghong;Giraldo, Juan Pablo
目前在植物中递送纳米材料的方法无法以高精度靶向特定的亚细胞区室，限制了我们设计植物功能的能力。我们展示了一个纳米级的平台，目标和生物化学物质提供纳米材料的植物光合细胞器（叶绿体）使用指导肽识别基序。量子点（QD）在低背景窗口的荧光发射允许共聚焦显微镜成像和定量检测植物细胞和细胞器中的元素分析。用β-环糊精分子篮的QD官能化使得能够装载和递送不同的化学品，并且用合理设计和保守的引导肽包被的纳米颗粒将其递送靶向叶绿体。肽生物识别提供了高递送效率和特异性的QD与化学货物在植物细胞中的叶绿体在体内（74.6 - 10.8%）和更具体的可调的叶绿体氧化还原功能的变化比单独的化学品。由生物识别基序引导的具有化学货物的纳米材料的靶向递送在植物生物学和生物工程、纳米颗粒-植物相互作用和纳米使能农业中具有广泛的纳米技术应用。将纳米材料靶向递送到亚细胞区室可以允许精确工程化植物功能。在此，桑塔纳等人表明，用合理设计的靶向肽官能化的量子点优先递送到叶绿体，在叶绿体中它们可用于调节细胞器氧化还原状态。
Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function. We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif. Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles. QD functionalization with beta -cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts. Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.610.8%) and more specific tunable changes of chloroplast redox function than chemicals alone. Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture. p id=Par Targeted delivery of nanomaterials to subcellular compartments could allow precision engineering of plant function. Here, Santana et al. show that quantum dots functionalized with a rationally-designed targeting peptide are preferentially delivered to chloroplasts where they can be used to tune organellar redox status.