Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation
Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem and organ boundary formation
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DOI：
10.1105/tpc.106.045716
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发表时间：
2006-11-01
期刊：
影响因子：
11.6
通讯作者：
Tasaka, Masao
中科院分区：
文献类型：
--
作者：
Hibara, Ken-ichiro;Karim, Md. Rezaul;Tasaka, Masao
在高等植物中，芽的整体结构高度依赖于胚性和腋生芽分生组织的活性，胚性和腋生芽分生组织分别产生于子叶和叶的近轴基部边界。在拟南芥中，杯形子叶基因CUC1、CUC2和CUC3的冗余功能调节胚芽分生组织的形成和子叶边界的特化。然而，它们在胚后发育中的功能重要性和关系却知之甚少。在这里，我们进行了广泛的分析的胚胎和胚胎后功能的三个CUC基因使用新分离的突变等位基因的多种组合。我们发现CUC2和CUC3，但不是CUC1，在腋生分生组织的形成和边界规格的各种胚后拍摄器官，如叶，茎，花梗的显着作用。在胚胎发生中，所有三个基因都做出了重要贡献，尽管CUC3似乎至少部分地具有与CUC1和CUC2不同的功能。CUC3和CUC2的功能与LATERAL SUPPLYOR的功能重叠，LATERAL SUPPLYOR以前被证明是腋生分生组织形成所必需的。我们的研究结果表明，冗余的，但部分不同的功能CUC1，CUC2，和CUC3是负责芽器官的边界和分生组织的形成在拟南芥的整个生活史。
Overall shoot architecture in higher plants is highly dependent on the activity of embryonic and axillary shoot meristems, which are produced from the basal adaxial boundaries of cotyledons and leaves, respectively. In Arabidopsis thaliana, redundant functions of the CUP-SHAPED COTYLEDON genes CUC1, CUC2, and CUC3 regulate embryonic shoot meristem formation and cotyledon boundary specification. Their functional importance and relationship in postembryonic development, however, is poorly understood. Here, we performed extensive analyses of the embryonic and postembryonic functions of the three CUC genes using multiple combinations of newly isolated mutant alleles. We found significant roles of CUC2 and CUC3, but not CUC1, in axillary meristem formation and boundary specification of various postembryonic shoot organs, such as leaves, stems, and pedicels. In embryogenesis, all three genes make significant contributions, although CUC3 appears to possess, at least partially, a distinct function from that of CUC1 and CUC2. The function of CUC3 and CUC2 overlaps that of LATERAL SUPPRESSOR, which was previously shown to be required for axillary meristem formation. Our results reveal that redundant but partially distinct functions of CUC1, CUC2, and CUC3 are responsible for shoot organ boundary and meristem formation throughout the life cycle in Arabidopsis.