Quantitative trait loci for grain fructan concentration in wheat (Triticum aestivum L.)
Quantitative trait loci for grain fructan concentration in wheat (Triticum aestivum L.)
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DOI：
10.1007/s00122-008-0811-6
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发表时间：
2008-09-01
影响因子：
5.4
通讯作者：
Mather, Diane E.
中科院分区：
文献类型：
--
作者：
Huynh, Bao-Lam;Wallwork, Hugh;Mather, Diane E.
果聚糖（低聚果糖）是益生元，被认为可以选择性地促进结肠双歧杆菌的生长，从而改善人类肠道健康。果聚糖存在于主食作物小麦的谷粒中。在本文报道的研究中，我们的目标是使用来自高果聚糖育种系Berkut和低果聚糖品种Krichauff杂交的双单倍体群体来检测和绘制影响小麦籽粒果聚糖浓度的基因座。果聚糖浓度测定谷物样品生长在两个地点在澳大利亚和哈萨克斯坦之一。果聚糖浓度在群体内变化很大，范围从0.6%至2.6%的粮食干重，是相当重复的，广义遗传力估计为0.71。利用528个分子标记构建的连锁图，在2B、3B、5A、6D和7A染色体上检测到数量性状位点（QTL）。其中，染色体6D和7A上的QTL具有最大的影响，分别解释了总表型方差的17%和27%，两者都具有来自Berkut的有利（高果聚糖浓度）等位基因。这些染色体区域在另一个作图群体Sokoll/Krichauff中具有相似的效应，有利等位基因来自Sokoll。因此，小麦籽粒果聚糖含量可以通过育种提高，分子标记可以有效地选择小麦基因组两个区域的有利等位基因。
Fructans (fructo-oligosaccharides) are prebiotics that are thought to selectively promote the growth of colonic bifidobacteria, thereby improving human gut health. Fructans are present in the grain of wheat, a staple food crop. In the research reported here, we aimed to detect and map loci affecting grain fructan concentration in wheat using a doubled-haploid population derived from a cross between a high-fructan breeding line, Berkut, and a low-fructan cultivar, Krichauff. Fructan concentration was measured in grain samples grown at two locations in Australia and one in Kazakhstan. Fructan concentration varied widely within the population, ranging from 0.6 to 2.6% of grain dry weight, and was quite repeatable, with broad-sense heritability estimated as 0.71. With a linkage map of 528 molecular markers, quantitative trait loci (QTLs) were detected on chromosomes 2B, 3B, 5A, 6D and 7A. Of these, the QTLs on chromosomes 6D and 7A had the largest effects, explaining 17 and 27% of the total phenotypic variance, respectively, both with the favourable (high-fructan concentration) alleles contributed from Berkut. These chromosome regions had similar effects in another mapping population, Sokoll/Krichauff, with the favourable alleles contributed from Sokoll. It is concluded that grain fructan concentration of wheat can be improved by breeding and that molecular markers could be used to select effectively for favourable alleles in two regions of the wheat genome.