In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy
In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy
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DOI：
10.1039/b419425j
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Knoll, W
中科院分区：
文献类型：
--
作者：
Liu, JY;Tian, SJ;Knoll, W
通过电化学阻抗谱 (EIS) 研究了金电极上肽核酸 (PNA) 和 6-巯基-1-己醇 (MCH) 混合单层上无标记 DNA 的原位杂交动力学，并用于区分完全互补的 DNA 和单碱基错配杂交体。
The in situ hybridization kinetics of label-free DNA on mixed monolayers of peptide nucleic acid (PNA) and 6-mercapto-1-hexanol (MCH) on Au electrodes was investigated by electrochemical impedance spectroscopy (EIS) and used to discriminate the fully complementary DNA from the single-base mismatched hybrids.