Hypocrea crystalligena sp nov., a common European species with a white-spored Trichoderma anamorph
Hypocrea crystalligena sp nov., a common European species with a white-spored Trichoderma anamorph
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DOI：
10.3852/mycologia.98.3.499
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发表时间：
2006-05-01
期刊：
影响因子：
2.8
通讯作者：
Druzhinina, Irina S.
中科院分区：
文献类型：
--
作者：
Jaklitsch, Walter M.;Komon, Monika;Druzhinina, Irina S.
新种：Hypocrea cystalligena（Hypocreales，Ascomycota，Fungi）被描述为一个全型，并基于综合的表型和系统发育方法，使用有性型和无性型形态，培养研究和系统发育标记（包括内部转录间隔区1和2）的分析来表征（ITS）和2），翻译延伸因子1-α编码基因（tef 1）的最后两个内含子，以及rpb 2基因的一部分，其编码第二大RNA聚合酶亚基。H.的基质crystigena与木霉组（Trichoderma sect. Trichoderma）的物种相似，但在几个方面不同，包括颜色、表面上存在白色晶体和小的子囊孢子。CMD上的菌落明显不同，分离时形成无色至白色晶体，黄色至棕色色素和具有透明分生孢子的无性型，表现出轮枝状至胶质枝状结构元件。ITSI和2序列表现出所有属特异性特征，但也包含允许开发物种诊断条形码的几个独特标志。根据rpb 2和tef 1部分序列的分析，ITcrystalligena与H. megalocitrina和H. psychrophila是它最近的邻居。所有这些种与H.枕状叶柠檬结
The new species Hypocrea ciystalligena (Hypocreales, Ascomycota, Fungi) is described as a holomorph and characterized based on an integrated phenotypic and phylogenetic approach, using teleomorph and anamorph morphologies, culture studies and analyses of phylogenetic markers including internal transcribed spacer 1 and 2 (ITS] and 2), two last introns of the translation elongation factor 1-alpha encoding gene (tef1), and a portion of the rpb2 gene, encoding the second largest RNA polymerase subunit. Stromata of H. crystalligena show similarities with those of species from Trichoderma sect. Trichoderma but differ in several respects, including color, presence of white crystals on the surface and small ascospores. Colonies on CMD appear distinct, form colorless to white crystals on isolation, a yellowish to brown pigment and an anamorph with hyaline conidia exhibiting verticillium-like to gliocladium-like structural elements. ITSI and 2 sequences exhibit all genus-specific features but also contain several unique hallmarks permitting development of a species-diagnostic barcode. Based on the analyses of partial rpb2 and tef1 sequences, IT crystalligena constitutes a separate evolutionary lineage with H. megalocitrina and H. psychrophila as its nearest neighbors. All these species form one phylogenetic clade with the H. pulvinatalH. citrina node.