MxRop1-MxrbohD1 interaction mediates ROS signaling in response to iron deficiency in the woody plant Malus xiaojinensis.
MxRop1-MxrbohD1 interaction mediates ROS signaling in response to iron deficiency in the woody plant Malus xiaojinensis.
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DOI：
10.1016/j.plantsci.2021.111071
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
L. Zhai;Chaohua Sun;Keting Li;Qiran Sun;Min Gao;Ting Wu;Xinzhong Zhang;Xuefeng Xu;Yi Wang;Zhenhai Han
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文献类型：
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作者：
L. Zhai;Chaohua Sun;Keting Li;Qiran Sun;Min Gao;Ting Wu;Xinzhong Zhang;Xuefeng Xu;Yi Wang;Zhenhai Han
缺铁影响作物产量和品质。植物的Rho（ROPs）参与植物体内的多种生理过程。缺铁胁迫下，尤其是多年生木本植物的缺铁胁迫，其生物学特性尚未得到很好的表征。本研究从小金海棠中克隆了ROP同源基因MxRop 1，并在拟南芥中进行了过量表达，结果表明该基因在缺铁胁迫下具有一定的功能。MxRop 1的过表达也增加了活性氧（ROS）水平。此外，活性状态的MxRop 1（CA-MxRop 1）与ROS合成基因MxrbohD 1的N端区域相互作用。当MxrbohD 1在苹果愈伤组织中过量表达时，H2 O2含量、鲜重和FCR活性显著增加，而ROS抑制剂显著抑制FCR活性，表明MxrbohD 1产生的ROS调节缺铁反应。此外，利用发根农杆菌转化法，MxrbohD 1基因在苹果根系中得到了高效表达，并提高了缺铁诱导基因的表达量和根系FCR活性。缺铁条件下，表现出轻微的叶片黄化表型。CA-MxRop 1和MxrbohD 1共表达可显著诱导ROS的产生。最后，我们推测MxRop 1与MxrbohD 1相互作用调节ROS介导的小金海棠缺铁适应性反应，这将为苹果耐缺铁植株的培育提供指导。
Iron (Fe) deficiency affects crop production and quality. Rho of plants (ROPs) involves in multiple physiological processes in plants. While it has not been well characterized under Fe deficiency, especially in perennial woody plants. In our study, we cloned ROP homologous geneMxRop1fromMalus xiaojinenesis, then overexpressed it inArabidopsis, showing enhanced plant tolerance to Fe deficiency, which demonstrated its gene function during this stress. Overexpression of MxRop1 also increased reactive oxygen species (ROS) levels. Moreover, active state of MxRop1 (CA-MxRop1) interacted with N-terminal region of MxrbohD1, one ROS synthesis gene. WhenMxrbohD1was overexpressed in apple calli, it showed significantly increased H2O2content, fresh weight and FCR activity, while ROS inhibitor application dramatically inhibited FCR activity, demonstrating ROS produced by MxrbohD1 regulated Fe deficiency responses. Furthermore, usingAgrobacterium rhizogenestransformation,MxrbohD1was overexpressed in apple roots, with increased expression of Fe deficiency-induced genes and increased root FCR activity. Under Fe deficiency, it exhibited slight leaf yellowing phenotype. Co-expression ofCA-MxRop1andMxrbohD1significantly induced ROS generation. Finally, we proposed that MxRop1 interacted with MxrbohD1 to modulate ROS mediated Fe deficiency adaptive responses inMalus xiaojinensis,which will provide a guidance of cultivation of Fe-deficiency tolerant apple plant.