Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR
Difterentially expressed genes of Tetrahymena thermophila in response to tributyltin (TBT) identified by suppression subtractive hybridization and real time quantitative PCR
复制标题
通过抑制消减杂交和实时定量PCR鉴定嗜热四膜虫对三丁基锡（TBT）反应的差异表达基因
DOI：
10.1016/j.aquatox.2006.11.005
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发表时间：
2007-02-15
影响因子：
4.5
通讯作者：
Wu, Yuxuan
中科院分区：
文献类型：
--
作者：
Feng, Lifang;Miao, Wei;Wu, Yuxuan
三丁基锡（TBT）在世界范围内被广泛用作油漆、农药、塑料稳定剂等，对水环境造成了严重的污染。然而，目前淡水中三丁基锡化合物的生物监测技术还很缺乏。构建了TBT诱导的嗜热四膜虫抑制性消减杂交文库，筛选出101个TBT诱导表达上调或下调的表达序列标签。由此发现了一系列与TBT毒性相关的基因，如谷胱甘肽-S-转移酶基因（下调）、质膜Ca ~（2+）ATP酶亚型3基因（上调）和NgoA基因（上调）。采用真实的实时荧光定量PCR检测不同浓度TBT（0.5-40 ppb）处理下的表达情况。鉴定了嗜热四膜虫响应TBT的差异表达基因，为利用rDNA诱导表达系统将四膜虫作为敏感、快速、方便的淡水TBT生物监测器奠定了基础。(c)2006 Elsevier B. V.保留所有权利。
Tributyltin (TBT) is widely used as antifouling paints, agriculture biocides, and plastic stabilizers around the world, resulting in great pollution problem in aquatic environments. However, it has been short of the biomonitor to detect TBT in freshwater. We constructed the suppression subtractive hybridization library of Tetrahymena thermophila exposed to TBT, and screened out 101 Expressed Sequence Tags whose expressions were significantly up- or down-regulated with TBT treatment. From this, a series of genes related to the TBT toxicity were discovered, such as glutathione-S-transferase gene (down-regulated), plasma membrane Ca2+ ATPase isoforms 3 gene (up-regulated) and NgoA (up-regulated). Furthermore, their expressions under different concentrations of TBT treatment (0.5-40 ppb) were detected by real time fluorescent quantitative PCR. The differentially expressed genes of T thermophila in response to TBT were identified, which provide the basic to make Tetrahymena as a sensitive, rapid and convenient TBT biomonitor in freshwater based on rDNA inducible expression system. (c) 2006 Elsevier B.V. All rights reserved.