Using chiral liquid chromatography quadrupole time-of-flight mass spectrometry for the analysis of pharmaceuticals and illicit drugs in surface and wastewater at the enantiomeric level
Using chiral liquid chromatography quadrupole time-of-flight mass spectrometry for the analysis of pharmaceuticals and illicit drugs in surface and wastewater at the enantiomeric level
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DOI：
10.1016/j.chroma.2012.06.012
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发表时间：
2012-08-03
影响因子：
4.1
通讯作者：
Kasprzyk-Hordern, B.
中科院分区：
文献类型：
--
作者：
Bagnall, J. P.;Evans, S. E.;Kasprzyk-Hordern, B.
本文首次提出并比较了两种手性LC-QTOF-MS方法（利用CBH和Chirobiotic V柱，纤维二糖水解酶和万古霉素作为手性选择剂）用于苯丙胺的定量。甲基苯丙胺、MDA（亚甲二氧基苯丙胺）、MDMA（亚甲二氧基甲基苯丙胺）、普萘洛尔。河水和污水中的阿替洛尔、美托洛尔、氟西汀和文拉法辛。使用手性柱Chirobiotic V观察到最低MDLs（河水和污水分别为0.3-5.0 ng L-1和1.3-15.1 ng L-1）。使用CBH柱观察到甲基苯丙胺和MDMA的MDLs较低除外。然而，CBH柱导致对映异构体的更好的分离度（对于苯丙胺，R-s = 2.5，相比之下，对于Chirobiotic V，R-s = 1.2）。两种方法的方法回收率通常>80%。使用MS/MS确认成功地鉴定了环境样品中检测和定量的药物和非法药物。在污水中，普萘洛尔、阿替洛尔和美托洛尔的总β-受体阻滞剂浓度平均为77.0、1091.0和3.6 ng L-1，因此EF（对映体分数）分别为0.43、0.55和0.54。在河水中，总普萘洛尔和阿替洛尔的平均定量为
This paper presents and compares for the first time two chiral LC-QTOF-MS methodologies (utilising CBH and Chirobiotic V columns with cellobiohydrolase and vancomycin as chiral selectors) for the quantification of amphetamine. methamphetamine, MDA (methylenedioxyamphetamine), MDMA (methylenedioxymethamphetamine), propranolol. atenolol, metoprolol, fluoxetine and venlafaxine in river water and sewage effluent. The lowest MDLs (0.3-5.0 ng L-1 and 1.3-15.1 ng L-1 for river water and sewage effluent respectively) were observed using the chiral column Chirobiotic V. This is with the exception of methamphetamine and MDMA which had lower MDLs using the CBH column. However, the CBH column resulted in better resolution of enantiomers (R-s = 2.5 for amphetamine compared with R-s = 1.2 with Chirobiotic V). Method recovery rates were typically >80% for both methodologies. Pharmaceuticals and illicit drugs detected and quantified in environmental samples were successfully identified using MS/MS confirmation. In sewage effluent, the total beta-blocker concentrations of propranolol, atenolol and metoprolol were on average 77.0, 1091.0 and 3.6 ng L-1 thus having EFs (Enantiomeric Fractions) of 0.43, 0.55 and 0.54 respectively. In river water, total propranolol and atenolol was quantified on average at