A virus-based biocatalyst
A virus-based biocatalyst
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DOI：
10.1038/nnano.2007.76
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发表时间：
2007-04-01
影响因子：
38.3
通讯作者：
Van Hest, Jan C. M.
中科院分区：
文献类型：
--
作者：
Carette, Noelle;Engelkamp, Hans;Van Hest, Jan C. M.
病毒颗粒可能是蛋白质自组装形成的最精确定义的纳米大小的物体。虽然它们的天然功能是储存和运输遗传物质，但它们最近已被用作矿化的支架和无机化合物包封的容器（1，2）。病毒的繁殖能力已被用于开发多功能分析方法，如噬菌体展示，用于选择和鉴定（生物）活性化合物（3）。迄今为止，自组装和复制的组合使用尚未用于构建催化系统。在这里，我们描述了一个自组装系统的基础上，植物病毒，其外壳蛋白基因修饰，以提供它与脂肪酶。使用单目标和散装催化研究，我们证明了病毒锚定脂肪酶分子的催化活性。这种锚定的生物催化剂，不像人造的负载型催化剂，具有在体内自我复制的能力，产生许多独立的催化活性副本。
Virus particles are probably the most precisely defined nanometre-sized objects that can be formed by protein self-assembly. Although their natural function is the storage and transport of genetic material, they have more recently been applied as scaffolds for mineralization and as containers for the encapsulation of inorganic compounds(1,2). The reproductive power of viruses has been used to develop versatile analytical methods, such as phage display, for the selection and identification of (bio) active compounds(3). To date, the combined use of self-assembly and reproduction has not been used for the construction of catalytic systems. Here we describe a self-assembled system based on a plant virus that has its coat protein genetically modified to provide it with a lipase enzyme. Using single-object and bulk catalytic studies, we prove that the virus-anchored lipase molecules are catalytically active. This anchored biocatalyst, unlike man-made supported catalysts, has the capability to reproduce itself in vivo, generating many independent catalytically active copies.