The role of conditioning film formation and surface chemical changes on Xylella fastidiosa adhesion and biofilm evolution
The role of conditioning film formation and surface chemical changes on Xylella fastidiosa adhesion and biofilm evolution
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DOI：
10.1016/j.jcis.2011.03.066
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发表时间：
2011-07-01
影响因子：
9.9
通讯作者：
Cotta, Monica A.
中科院分区：
文献类型：
--
作者：
Lorite, Gabriela S.;Rodrigues, Carolina M.;Cotta, Monica A.
生物膜是复杂的微生物群落，具有重要的生物学功能，包括增强对抗菌剂等外部因素的抵抗力。众所周知，生物膜的形成强烈依赖于底物的性质，包括疏水性/亲水性、结构和粗糙度。(大分子)分子在底物上的吸附，也被称为调理膜，改变了表面的物理化学性质，影响了细菌的黏附。在本研究中，我们研究了在不同表面(玻璃和硅)上长春枯萎病(PW)培养基条件化成膜所引起的物理化学变化及其对X.Fastidiosa生物膜形成的影响。接触角测量表明，几个小时后，薄膜的形成降低了玻璃和硅的表面亲水性。原子力显微镜(AFM)图像显示，随着调节膜的形成，玻璃表面粗糙度显著降低。经过一段时间后，在AFM液体细胞中观察到玻璃上的第一层金黄色葡萄球菌生物膜，与测定的亲水性变化相似。此外，衰减全反射傅里叶变换红外光谱(ATR-FTIR)支持AFM观察，因为PW吸收光谱随着时间的增加而增加，表明磷酸基团的贡献更大。虽然疏水和粗糙的表面通常被认为可以增加细菌细胞的附着，但我们的结果表明，这些特性并不像PW调节膜形成的表面官能团那样重要，对于X.tentidiosa的附着和生物膜的发展。(C)2011 Elsevier Inc.保留所有权利。
Biofilms are complex microbial communities with important biological functions including enhanced resistance against external factors like antimicrobial agents. The formation of a biofilm is known to be strongly dependent on substrate properties including hydrophobicity/hydrophilicity, structure, and roughness. The adsorption of (macro)molecules on the substrate, also known as conditioning film, changes the physicochemical properties of the surface and affects the bacterial adhesion. In this study, we investigate the physicochemical changes caused by Periwinkle wilt (PW) culture medium conditioning film formation on different surfaces (glass and silicon) and their effect on X. fastidiosa biofilm formation. Contact angle measurements have shown that the film formation decreases the surface hydrophilicity degree of both glass and silicon after few hours. Atomic force microscopy (AFM) images show the glass surface roughness is drastically reduced with conditioning film formation. First-layer X. fastidiosa biofilm on glass was observed in the AFM liquid cell after a period of time similar to that determined for the hydrophilicity changes. In addition, attenuation total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy supports the AFM observation, since the PW absorption spectra increases with time showing a stronger contribution from the phosphate groups. Although hydrophobic and rough surfaces are commonly considered to increase bacteria cell attachment, our results suggest that these properties are not as important as the surface functional groups resulting from PW conditioning film formation for X. fastidiosa adhesion and biofilm development. (c) 2011 Elsevier Inc. All rights reserved.