Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique
Identification of Differentially Expressed Genes in Different Types of Broiler Skeletal Muscle Fibers Using the RNA-seq Technique
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利用 RNA-seq 技术鉴定不同类型肉鸡骨骼肌纤维中差异表达的基因
DOI：
10.1155/2020/9478949
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发表时间：
2020-07-06
影响因子：
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通讯作者：
Zhao, Ayong
中科院分区：
文献类型：
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作者：
Wang, Han;Shen, Zhonghao;Zhao, Ayong
肌纤维类型的差异对肉仔鸡肌肉发育和肉品质有重要影响。目前，肉鸡骨骼肌纤维型转化的分子调控机制尚不清楚。本研究利用RNA-seq技术对肉鸡胸肌和腿肌组织中差异表达基因进行了分析。总共确定了767名DEG。与腿肌相比，胸肌中有429个基因表达上调，338个基因表达下调。基因本体（GO）富集表明这些DEG主要参与细胞过程、单个生物过程、细胞和细胞组分，以及结合和催化活性。KEGG分析表明，共有230个DEG被映射到126个KEGG途径，并显着富集在糖酵解/糖异生，淀粉和蔗糖代谢，胰岛素信号转导途径和氨基酸的生物合成的四个途径。采用实时定量逆转录聚合酶链反应（qRT-PCR）验证7个DEG的差异表达，结果与RNA-seq数据一致。此外，鸡骨骼肌细胞中MyHC亚型的表达谱显示，随着分化时间的延长，快肌纤维亚基（IIA型和IIB型）的表达逐渐增加，而慢肌纤维亚基（I型）在分化4 d后呈下降趋势。本研究筛选的差异基因将为进一步了解肉鸡骨骼肌纤维转化的分子机制提供一些新的思路。
The difference in muscle fiber types is very important to the muscle development and meat quality of broilers. At present, the molecular regulation mechanisms of skeletal muscle fiber-type transformation in broilers are still unclear. In this study, differentially expressed genes between breast and leg muscles in broilers were analyzed using RNA-seq. A total of 767 DEGs were identified. Compared with leg muscle, there were 429 upregulated genes and 338 downregulated genes in breast muscle. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in cellular processes, single organism processes, cells, and cellular components, as well as binding and catalytic activity. KEGG analysis shows that a total of 230 DEGs were mapped to 126 KEGG pathways and significantly enriched in the four pathways of glycolysis/gluconeogenesis, starch and sucrose metabolism, insulin signalling pathways, and the biosynthesis of amino acids. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to verify the differential expression of 7 selected DEGs, and the results were consistent with RNA-seq data. In addition, the expression profile of MyHC isoforms in chicken skeletal muscle cells showed that with the extension of differentiation time, the expression of fast fiber subunits (types IIA and IIB) gradually increased, while slow muscle fiber subunits (type I) showed a downward trend after 4 days of differentiation. The differential genes screened in this study will provide some new ideas for further understanding the molecular mechanism of skeletal muscle fiber transformation in broilers.