Incidence of a new sex–ratio–distorting endosymbiotic bacterium among arthropods
Incidence of a new sex–ratio–distorting endosymbiotic bacterium among arthropods
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DOI：
10.1098/rspb.2003.2425
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发表时间：
2003-09
期刊：
影响因子：
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通讯作者：
A. Weeks;R. Velten;R. Stouthamer
中科院分区：
文献类型：
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作者：
A. Weeks;R. Velten;R. Stouthamer
现在已知，许多细胞内微生物会导致宿主的生殖异常和其他现象。内共生沃尔巴克氏菌是这些生殖操纵者中最著名的，因为它在节肢动物中的发病率极高，而且它所引起的寄主效应也不同。然而，最近的证据表明，另一种胞内细菌，一种类似细胞吞噬的有机体(CLO)，也可能在其宿主中诱导几种生殖效应。在这里，我们提出了第一次调查节肢动物宿主感染CLO。我们使用一种灵敏的半套式聚合酶链式反应方法对20个节肢动物目中的223个物种进行了CLO和Wolbachia的感染筛查。结果表明，虽然没有沃尔巴克氏菌流行，但CLO侵染了相当数量的节肢动物宿主(约7.2%)。此外，在7种节肢动物个体中还发现了CLO和Wolbachia的双重感染。对CLO的16S rDNA区域的测序分析表明，CLO菌株存在水平传播的证据。我们参考未来对细胞内微生物诱导的宿主效应的研究来讨论这些结果。
Many intracellular micro–organisms are now known to cause reproductive abnormalities and other phenomena in their hosts. The endosymbiont Wolbachia is the best known of these reproductive manipulators owing to its extremely high incidence among arthropods and the diverse host effects it has been implicated as causing. However, recent evidence suggests that another intracellular bacterium, a Cytophaga–like organism (CLO), may also induce several reproductive effects in its hosts. Here, we present the first survey of arthropod hosts for infection by the CLO. We use a sensitive hemi–nested polymerase chain reaction method to screen 223 species from 20 arthropod orders for infection by the CLO and Wolbachia. The results indicate that, although not as prevalent as Wolbachia, the CLO infects a significant number of arthropod hosts (ca. 7.2%). In addition, double infections of the CLO and Wolbachia were found in individuals of seven arthropod species. Sequencing analysis of the 16S rDNA region of the CLO indicates evidence for horizontal transmission of the CLO strains. We discuss these results with reference to future studies on host effects induced by intracellular micro–organisms.